PMID- 10644674
OWN - NLM
STAT- MEDLINE
DCOM- 20000229
LR  - 20190606
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 275
IP  - 4
DP  - 2000 Jan 28
TI  - Structural analysis of glycosaminoglycans in Drosophila and Caenorhabditis
      elegans and demonstration that tout-velu, a Drosophila gene related to EXT tumor 
      suppressors, affects heparan sulfate in vivo.
PG  - 2269-75
AB  - We have devised a sensitive method for the isolation and structural analysis of
      glycosaminoglycans from two genetically tractable model organisms, the fruit fly,
      Drosophila melanogaster, and the nematode, Caenorhabditis elegans. We detected
      chondroitin/chondroitin sulfate- and heparan sulfate-derived disaccharides in
      both organisms. Chondroitinase digestion of glycosaminoglycans from adult
      Drosophila produced both nonsulfated and 4-O-sulfated unsaturated disaccharides, 
      whereas only unsulfated forms were detected in C. elegans. Heparin lyases
      released disaccharides bearing N-, 2-O-, and 6-O-sulfated species, including
      mono-, di-, and trisulfated forms. We observed tissue- and stage-specific
      differences in both chondroitin sulfate and heparan sulfate composition in
      Drosophila. We have also applied these methods toward the analysis of tout-velu, 
      an EXT-related gene in Drosophila that controls the tissue distribution of the
      growth factor Hedgehog. The proteins encoded by the vertebrate tumor suppressor
      genes EXT1 and 2, show heparan sulfate co-polymerase activity, and it has been
      proposed that tout-velu affects Hedgehog activity via its role in heparan sulfate
      biosynthesis. Analysis of total glycosaminoglycans from tout-velu mutant larvae
      show marked reductions in heparan sulfate but not chondroitin sulfate, consistent
      with its proposed function as a heparan sulfate co-polymerase.
FAU - Toyoda, H
AU  - Toyoda H
AD  - Department of Molecular Biology, University of Arizona, Tucson, Arizona 85721,
      USA.
FAU - Kinoshita-Toyoda, A
AU  - Kinoshita-Toyoda A
FAU - Selleck, S B
AU  - Selleck SB
LA  - eng
GR  - GM-25243/GM/NIGMS NIH HHS/United States
GR  - GM-54832/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (Drosophila Proteins)
RN  - 0 (Membrane Proteins)
RN  - 0 (TTV protein, Drosophila)
RN  - 9050-30-0 (Heparitin Sulfate)
SB  - IM
MH  - Animals
MH  - Caenorhabditis elegans/*metabolism
MH  - Carbohydrate Conformation
MH  - Cattle
MH  - Chromatography, High Pressure Liquid
MH  - Drosophila/genetics/growth & development/*metabolism
MH  - *Drosophila Proteins
MH  - *Genes, Tumor Suppressor
MH  - Heparitin Sulfate/*metabolism
MH  - Membrane Proteins/*genetics
EDAT- 2000/01/25 09:00
MHDA- 2000/03/04 09:00
CRDT- 2000/01/25 09:00
PHST- 2000/01/25 09:00 [pubmed]
PHST- 2000/03/04 09:00 [medline]
PHST- 2000/01/25 09:00 [entrez]
AID - 10.1074/jbc.275.4.2269 [doi]
PST - ppublish
SO  - J Biol Chem. 2000 Jan 28;275(4):2269-75. doi: 10.1074/jbc.275.4.2269.