PMID- 10644447 OWN - NLM STAT- MEDLINE DCOM- 20000302 LR - 20201208 IS - 0888-7543 (Print) IS - 0888-7543 (Linking) VI - 62 IP - 3 DP - 1999 Dec 15 TI - Molecular cloning and chromosomal mapping of the human homologue of MYB binding protein (P160) 1A (MYBBP1A) to 17p13.3. PG - 483-9 AB - We have previously isolated and characterized murine MYB binding protein (p160) 1a, a protein that specifically interacts with the leucine zipper motif within the negative regulatory domain of the c-Myb proto-oncoprotein. We now describe the molecular cloning of the human MYBBP1A cDNA and chromosomal localization to 17p13.3 by fluorescence in situ hybridization analysis. Given the likely presence of a tumor suppressor gene (or genes) within this region of chromosome 17, the position of MYBBP1A was further mapped by radiation hybrid analysis and was found to lie between markers D17S1828 and D17S938. A P1 artificial chromosome clone containing the 5' region of MYBBP1A was isolated and indicates a physical linkage between MYBBP1A and the 15-lipoxygenase gene (ALOX15). A novel, polymorphic (CA)(25) dinucleotide repeat was also isolated from this PAC and may serve as a useful marker for MYBBP1A and this region of chromosome 17. CI - Copyright 1999 Academic Press. FAU - Keough, R AU - Keough R AD - Hanson Centre for Cancer Research, Institute of Medical and Veterinary Science, Adelaide, South Australia, 5000, Australia. FAU - Woollatt, E AU - Woollatt E FAU - Crawford, J AU - Crawford J FAU - Sutherland, G R AU - Sutherland GR FAU - Plummer, S AU - Plummer S FAU - Casey, G AU - Casey G FAU - Gonda, T J AU - Gonda TJ LA - eng SI - GENBANK/AF147709 PT - Journal Article PT - Research Support, Non-U.S. Gov't PL - United States TA - Genomics JT - Genomics JID - 8800135 RN - 0 (Carrier Proteins) RN - 0 (DNA-Binding Proteins) RN - 0 (Genetic Markers) RN - 0 (MYBBP1A protein, human) RN - 0 (Nuclear Proteins) RN - 0 (Nucleocytoplasmic Transport Proteins) RN - 0 (RNA-Binding Proteins) RN - 0 (Transcription Factors) SB - IM MH - Alleles MH - Amino Acid Sequence MH - Base Sequence MH - Blotting, Northern MH - Carrier Proteins/*genetics MH - Chromosomes, Human, Pair 17/*genetics MH - Cloning, Molecular MH - DNA-Binding Proteins MH - Dinucleotide Repeats MH - Genetic Markers MH - Humans MH - In Situ Hybridization, Fluorescence MH - Molecular Sequence Data MH - Nuclear Proteins/*genetics MH - Nucleocytoplasmic Transport Proteins MH - Organ Specificity/genetics MH - *Physical Chromosome Mapping MH - RNA-Binding Proteins MH - Sequence Analysis, DNA MH - *Sequence Homology, Amino Acid MH - Terminology as Topic MH - Transcription Factors EDAT- 2000/01/25 09:00 MHDA- 2000/03/04 09:00 CRDT- 2000/01/25 09:00 PHST- 2000/01/25 09:00 [pubmed] PHST- 2000/03/04 09:00 [medline] PHST- 2000/01/25 09:00 [entrez] AID - 10.1006/geno.1999.6035 [doi] AID - S0888-7543(99)96035-4 [pii] PST - ppublish SO - Genomics. 1999 Dec 15;62(3):483-9. doi: 10.1006/geno.1999.6035.