PMID- 10644440
OWN - NLM
STAT- MEDLINE
DCOM- 20000302
LR  - 20071114
IS  - 0888-7543 (Print)
IS  - 0888-7543 (Linking)
VI  - 62
IP  - 3
DP  - 1999 Dec 15
TI  - Characterization of the mouse Xpf DNA repair gene and differential expression
      during spermatogenesis.
PG  - 427-35
AB  - The human XPF protein, an endonuclease subunit essential for DNA excision repair,
      may also function in homologous recombination. To investigate a possible link
      between mammalian XPF and recombination that occurs during meiosis, we isolated, 
      characterized, and determined an expression profile for the mouse Xpf gene. The
      predicted mouse XPF protein, encoded by a 3.4-kb cDNA, contains 917 amino acids
      and is 86% identical to human XPF. Appreciable similarity also exists between
      mouse XPF and homologous proteins in budding yeast (Rad1), fission yeast (Rad16),
      and fruit fly (Mei-9), all of which have dual functions in excision repair and
      recombination. Sequence analysis of the 38.3-kb Xpf gene, localized to a region
      in proximal mouse chromosome 16, revealed greater than 72% identity to human XPF 
      in 16 regions. Of these conserved elements, 11 were exons and 5 were noncoding
      sequence within introns. Xpf transcript and protein levels were specifically
      elevated in adult mouse testis. Moreover, increased levels of Xpf and Ercc1 mRNAs
      correlated with meiotic and early postmeiotic spermatogenic cells. These results 
      support a distinct role for the XPF/ERCC1 junction-specific endonuclease during
      meiosis, most likely in the resolution of heteroduplex intermediates that arise
      during recombination.
CI  - Copyright 1999 Academic Press.
FAU - Shannon, M
AU  - Shannon M
AD  - Molecular and Structural Biology Division, Lawrence Livermore National
      Laboratory, Livermore, California 94550, USA.
FAU - Lamerdin, J E
AU  - Lamerdin JE
FAU - Richardson, L
AU  - Richardson L
FAU - McCutchen-Maloney, S L
AU  - McCutchen-Maloney SL
FAU - Hwang, M H
AU  - Hwang MH
FAU - Handel, M A
AU  - Handel MA
FAU - Stubbs, L
AU  - Stubbs L
FAU - Thelen, M P
AU  - Thelen MP
LA  - eng
SI  - GENBANK/AF189285
GR  - GM52120/GM/NIGMS NIH HHS/United States
GR  - HD31376/HD/NICHD NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Genomics
JT  - Genomics
JID - 8800135
RN  - 0 (DNA, Complementary)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Proteins)
RN  - 0 (xeroderma pigmentosum group F protein)
RN  - EC 3.1.- (Endonucleases)
RN  - EC 3.1.- (Ercc1 protein, mouse)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Chromosome Mapping
MH  - Cloning, Molecular
MH  - DNA Repair
MH  - DNA, Complementary/analysis
MH  - DNA-Binding Proteins/*biosynthesis/*genetics
MH  - *Endonucleases
MH  - *Gene Expression
MH  - Germ Cells/metabolism
MH  - Male
MH  - Mice
MH  - Molecular Sequence Data
MH  - Organ Specificity/genetics
MH  - Proteins/metabolism
MH  - Sequence Homology, Amino Acid
MH  - Spermatogenesis/*genetics
MH  - Testis/metabolism
EDAT- 2000/01/25 09:00
MHDA- 2000/03/04 09:00
CRDT- 2000/01/25 09:00
PHST- 2000/01/25 09:00 [pubmed]
PHST- 2000/03/04 09:00 [medline]
PHST- 2000/01/25 09:00 [entrez]
AID - 10.1006/geno.1999.6016 [doi]
AID - S0888-7543(99)96016-0 [pii]
PST - ppublish
SO  - Genomics. 1999 Dec 15;62(3):427-35. doi: 10.1006/geno.1999.6016.