PMID- 10644367
OWN - NLM
STAT- MEDLINE
DCOM- 20000302
LR  - 20190508
IS  - 0022-538X (Print)
IS  - 0022-538X (Linking)
VI  - 74
IP  - 4
DP  - 2000 Feb
TI  - A role for SKIP in EBNA2 activation of CBF1-repressed promoters.
PG  - 1939-47
AB  - EBNA2 is essential for Epstein-Barr virus (EBV) immortalization of B lymphocytes.
      EBNA2 functions as a transcriptional activator and targets responsive promoters
      through interaction with the cellular DNA binding protein CBF1. We have examined 
      the mechanism whereby EBNA2 overcomes CBF1-mediated transcriptional repression. A
      yeast two-hybrid screen performed using CBF1 as the bait identified a protein,
      SKIP, which had not previously been recognized as a CBF1-associated protein.
      Protein-protein interaction assays demonstrated contacts between SKIP and the
      SMRT, CIR, Sin3A, and HDAC2 proteins of the CBF1 corepressor complex.
      Interestingly, EBNA2 also interacted with SKIP in glutathione S-transferase
      affinity and mammalian two-hybrid assays and colocalized with SKIP in
      immunofluorescence assays. Interaction with SKIP was not affected by mutation of 
      EBNA2 conserved region 6, the CBF1 interaction region, but was abolished by
      mutation of conserved region 5. Mutation of conserved region 5 also severely
      impaired EBNA2 activation of a reporter containing CBF1 binding sites. Thus,
      interaction with both CBF1 and SKIP is necessary for efficient promoter
      activation by EBNA2. A model is presented in which EBNA2 competes with the
      SMRT-corepressor complex for contacts on SKIP and CBF1.
FAU - Zhou, S
AU  - Zhou S
AD  - Department of Pharmacology and Molecular Sciences, Johns Hopkins School of
      Medicine, Baltimore, Maryland 21205, USA.
FAU - Fujimuro, M
AU  - Fujimuro M
FAU - Hsieh, J J
AU  - Hsieh JJ
FAU - Chen, L
AU  - Chen L
FAU - Hayward, S D
AU  - Hayward SD
LA  - eng
GR  - R01 CA042245/CA/NCI NIH HHS/United States
GR  - R01 CA42245/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Virol
JT  - Journal of virology
JID - 0113724
RN  - 0 (CIR1 protein, human)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (EBNA-2 protein, Human herpesvirus 4)
RN  - 0 (Epstein-Barr Virus Nuclear Antigens)
RN  - 0 (Immunoglobulin J Recombination Signal Sequence-Binding Protein)
RN  - 0 (NCOR2 protein, human)
RN  - 0 (Nuclear Proteins)
RN  - 0 (Nuclear Receptor Co-Repressor 2)
RN  - 0 (Nuclear Receptor Coactivators)
RN  - 0 (RBPJ protein, human)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 0 (Repressor Proteins)
RN  - 0 (SIN3A transcription factor)
RN  - 0 (SNW1 protein, human)
RN  - 0 (Transcription Factors)
RN  - 0 (Viral Proteins)
RN  - EC 3.5.1.98 (Histone Deacetylase 2)
RN  - EC 3.5.1.98 (Histone Deacetylases)
SB  - IM
MH  - Amino Acid Sequence
MH  - Binding Sites
MH  - Cell Line, Transformed
MH  - DNA-Binding Proteins/genetics/*metabolism
MH  - *Epstein-Barr Virus Nuclear Antigens
MH  - *Gene Expression Regulation
MH  - HeLa Cells
MH  - Histone Deacetylase 2
MH  - Histone Deacetylases/metabolism
MH  - Humans
MH  - Immunoglobulin J Recombination Signal Sequence-Binding Protein
MH  - Molecular Sequence Data
MH  - Nuclear Proteins/genetics/metabolism/*physiology
MH  - Nuclear Receptor Co-Repressor 2
MH  - Nuclear Receptor Coactivators
MH  - *Promoter Regions, Genetic
MH  - Recombinant Fusion Proteins/genetics/metabolism
MH  - Repressor Proteins/metabolism
MH  - Transcription Factors
MH  - Transfection
MH  - Viral Proteins/genetics/*metabolism
PMC - PMC111672
EDAT- 2000/01/22 09:00
MHDA- 2000/03/04 09:00
CRDT- 2000/01/22 09:00
PHST- 2000/01/22 09:00 [pubmed]
PHST- 2000/03/04 09:00 [medline]
PHST- 2000/01/22 09:00 [entrez]
AID - 10.1128/jvi.74.4.1939-1947.2000 [doi]
PST - ppublish
SO  - J Virol. 2000 Feb;74(4):1939-47. doi: 10.1128/jvi.74.4.1939-1947.2000.