PMID- 10644344
OWN - NLM
STAT- MEDLINE
DCOM- 20000302
LR  - 20190508
IS  - 0022-538X (Print)
IS  - 0022-538X (Linking)
VI  - 74
IP  - 4
DP  - 2000 Feb
TI  - Hepatitis C virus core protein interacts with 14-3-3 protein and activates the
      kinase Raf-1.
PG  - 1736-41
AB  - Persistent hepatitis C virus (HCV) infection is a major cause of chronic liver
      dysfunction in humans and is epidemiologically closely associated with the
      development of human hepatocellular carcinoma. Among HCV components, core protein
      has been reported to be implicated in cell growth regulation both in vitro and in
      vivo, although mechanisms explaining those effects are still unclear. In the
      present study, we identified that members of the 14-3-3 protein family associate 
      with HCV core protein. 14-3-3 protein bound to HCV core protein in a
      phosphoserine-dependent manner. Introduction of HCV core protein caused a
      substantial increase in Raf-1 kinase activity in HepG2 cells and in a yeast
      genetic assay. Furthermore, the HCV core-14-3-3 interaction was essential for
      Raf-1 kinase activation by HCV core protein. These results suggest that HCV core 
      protein may represent a novel type of Raf-1 kinase-activating protein through its
      interaction with 14-3-3 protein and may contribute to hepatocyte growth
      regulation.
FAU - Aoki, H
AU  - Aoki H
AD  - Department of Experimental Pathology, Cancer Institute, Japanese Foundation for
      Cancer Research, 1-37-1 Kami-Ikebukuro, Toshima-ku, Tokyo 170-8455, Japan.
FAU - Hayashi, J
AU  - Hayashi J
FAU - Moriyama, M
AU  - Moriyama M
FAU - Arakawa, Y
AU  - Arakawa Y
FAU - Hino, O
AU  - Hino O
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Virol
JT  - Journal of virology
JID - 0113724
RN  - 0 (14-3-3 Proteins)
RN  - 0 (Protein Isoforms)
RN  - 0 (Proteins)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 0 (Viral Core Proteins)
RN  - 0 (nucleocapsid protein, Hepatitis C virus)
RN  - 17885-08-4 (Phosphoserine)
RN  - 452VLY9402 (Serine)
RN  - EC 1.14.16.2 (Tyrosine 3-Monooxygenase)
RN  - EC 2.7.11.1 (Proto-Oncogene Proteins c-raf)
RN  - EC 2.7.11.11 (Cyclic AMP-Dependent Protein Kinases)
RN  - EC 2.7.11.13 (Protein Kinase C)
SB  - IM
MH  - 14-3-3 Proteins
MH  - Binding Sites
MH  - Cyclic AMP-Dependent Protein Kinases/metabolism
MH  - Enzyme Activation
MH  - *Hepacivirus
MH  - Humans
MH  - Phosphorylation
MH  - Phosphoserine/metabolism
MH  - Protein Isoforms/metabolism
MH  - Protein Kinase C/metabolism
MH  - Proteins/genetics/*metabolism
MH  - Proto-Oncogene Proteins c-raf/genetics/*metabolism
MH  - Recombinant Fusion Proteins/genetics/metabolism
MH  - Saccharomyces cerevisiae
MH  - Serine
MH  - Tumor Cells, Cultured
MH  - *Tyrosine 3-Monooxygenase
MH  - Viral Core Proteins/genetics/*metabolism
PMC - PMC111649
EDAT- 2000/01/22 09:00
MHDA- 2000/03/04 09:00
CRDT- 2000/01/22 09:00
PHST- 2000/01/22 09:00 [pubmed]
PHST- 2000/03/04 09:00 [medline]
PHST- 2000/01/22 09:00 [entrez]
AID - 10.1128/jvi.74.4.1736-1741.2000 [doi]
PST - ppublish
SO  - J Virol. 2000 Feb;74(4):1736-41. doi: 10.1128/jvi.74.4.1736-1741.2000.