PMID- 10643790
OWN - NLM
STAT- MEDLINE
DCOM- 20000222
LR  - 20190701
IS  - 0304-3940 (Print)
IS  - 0304-3940 (Linking)
VI  - 278
IP  - 1-2
DP  - 2000 Jan 7
TI  - Hypoxia induces activation of a N-methyl-D-aspartate glutamate receptor-protein
      kinase C pathway in the dorsocaudal brainstem of the conscious rat.
PG  - 17-20
AB  - To study in vivo phosphorylation of N-methyl-D-aspartate (NMDA) glutamate
      receptors and the recruitment of protein kinase C isoforms during acute hypoxia, 
      dorsocaudal brainstem lysates were harvested from conscious rats exposed to
      either room air or hypoxia (10% O2 for 5 and 15 min). Increased phosphorylation
      of the NR-1 subunit at serine residue 896 occurred during hypoxia and was blocked
      by pre-treatment with MK-801. Immunoblots of soluble and particulate fractions
      revealed subcellular translocation for PKC-beta, -gamma, -delta, -epsilon, and
      -iota during hypoxia with no changes in PKC-alpha, -mu, and -zeta. Translocation 
      of PKC-beta, -delta and -epsilon was selectively attenuated following MK-801. We 
      demonstrate that hypoxia leads to PKC-mediated activation of NMDA receptors in
      the brainstem, and that PKC-beta, -delta and -epsilon are the most likely
      candidates for NR-1 phosphorylation.
FAU - Simakajornboon, N
AU  - Simakajornboon N
AD  - Department of Pediatrics, Constance S. Kaufman Pediatric Pulmonary Research
      Laboratory, Tulane University School of Medicine, New Orleans, LA 70112, USA.
FAU - Gozal, E
AU  - Gozal E
FAU - Gozal, Y M
AU  - Gozal YM
FAU - Gozal, D
AU  - Gozal D
LA  - eng
GR  - HD-01072/HD/NICHD NIH HHS/United States
GR  - HL-65270/HL/NHLBI NIH HHS/United States
GR  - MCJ-229163/PHS HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Ireland
TA  - Neurosci Lett
JT  - Neuroscience letters
JID - 7600130
RN  - 0 (Excitatory Amino Acid Antagonists)
RN  - 0 (Isoenzymes)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (Receptors, N-Methyl-D-Aspartate)
RN  - 6LR8C1B66Q (Dizocilpine Maleate)
RN  - EC 2.7.11.13 (Protein Kinase C)
SB  - IM
MH  - Animals
MH  - Biological Transport
MH  - Brain Stem/*metabolism
MH  - Consciousness
MH  - Dizocilpine Maleate/pharmacology
MH  - Enzyme Activation
MH  - Excitatory Amino Acid Antagonists/*pharmacology
MH  - Hypoxia/*metabolism
MH  - Isoenzymes/*physiology
MH  - Nerve Tissue Proteins/*physiology
MH  - Phosphorylation
MH  - Protein Kinase C/*physiology
MH  - *Protein Processing, Post-Translational
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Receptors, N-Methyl-D-Aspartate/*metabolism
MH  - *Signal Transduction
EDAT- 2000/01/22 09:00
MHDA- 2000/02/26 09:00
CRDT- 2000/01/22 09:00
PHST- 2000/01/22 09:00 [pubmed]
PHST- 2000/02/26 09:00 [medline]
PHST- 2000/01/22 09:00 [entrez]
AID - S0304-3940(99)00887-3 [pii]
AID - 10.1016/s0304-3940(99)00887-3 [doi]
PST - ppublish
SO  - Neurosci Lett. 2000 Jan 7;278(1-2):17-20. doi: 10.1016/s0304-3940(99)00887-3.