PMID- 10643692
OWN - NLM
STAT- MEDLINE
DCOM- 20000211
LR  - 20140603
VI  - 23
IP  - 12
DP  - 1999 Dec
TI  - Cloning and mRNA tissue distribution of human PPARgamma coactivator-1.
PG  - 1327-32
AB  - OBJECTIVES: To determine human PPARgamma coactivator-1 (PGC-1) amino acid
      sequence and to study PGC-1 mRNA tissue distribution. PGC-1 is a novel
      transcriptional coactivator of nuclear receptors that may play a role in the
      control of thermogenesis. SUBJECTS: Subcutaneous adipose tissue was obtained from
      six obese and five lean male subjects. Vastus lateralis skeletal muscle was
      obtained from seven lean and six obese subjects undergoing a 5-day severe calorie
      restriction. Other tissue biopsies were from nonobese nondiabetic subjects.
      METHODS: Human PGC-1 was cloned from a skeletal muscle cDNA library. A reverse
      transcription-competitive polymerase chain reaction assay was developed to
      determine PGC-1 mRNA levels in human tissues. RESULTS: The human amino acid
      sequence showed 95% identity with mouse PGC-1. PGC-1 mRNA was expressed at very
      low levels in the small and large intestines and white adipose tissue. Heart,
      kidney, liver and skeletal muscle showed higher mRNA levels. The degree of
      obesity did not affect PGC-1 mRNA levels in adipose tissue while lean subjects
      expressed more PGC-1 mRNA than obese subjects in skeletal muscle. A 5-day severe 
      calorie restriction induced PGC-1 mRNA expression in skeletal muscle of obese but
      not of lean subjects. CONCLUSION: PGC-1 shows a restricted tissue expression that
      suggests a tissue-specific role in the control of gene transcription and possible
      interaction with various members of the PPAR family. The lower expression of
      skeletal muscle PGC-1 in obesity could contribute to an alteration of
      mitochondrial gene expression.
FAU - Larrouy, D
AU  - Larrouy D
AD  - INSERM Unit 317, Institut Louis Bugnard, Universite Paul Sabatier, Hopital
      Rangueil, Toulouse, France.
FAU - Vidal, H
AU  - Vidal H
FAU - Andreelli, F
AU  - Andreelli F
FAU - Laville, M
AU  - Laville M
FAU - Langin, D
AU  - Langin D
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Int J Obes Relat Metab Disord
JT  - International journal of obesity and related metabolic disorders : journal of the
      International Association for the Study of Obesity
JID - 9313169
RN  - 0 (RNA, Messenger)
RN  - 0 (Transcription Factors)
RN  - 0 (peroxisome-proliferator-activated receptor-gamma coactivator-1)
SB  - IM
MH  - Adipose Tissue/metabolism
MH  - Adult
MH  - Amino Acid Sequence
MH  - Body Temperature Regulation
MH  - Case-Control Studies
MH  - Cloning, Molecular
MH  - Fasting
MH  - Female
MH  - Gene Expression Regulation
MH  - Gene Library
MH  - Humans
MH  - Male
MH  - Middle Aged
MH  - Molecular Sequence Data
MH  - Muscle, Skeletal/metabolism
MH  - Obesity/*metabolism
MH  - RNA, Messenger/analysis/biosynthesis
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Tissue Distribution
MH  - Transcription Factors/chemistry/genetics/*metabolism
EDAT- 2000/01/22 09:00
MHDA- 2000/02/19 09:00
CRDT- 2000/01/22 09:00
PHST- 2000/01/22 09:00 [pubmed]
PHST- 2000/02/19 09:00 [medline]
PHST- 2000/01/22 09:00 [entrez]
PST - ppublish
SO  - Int J Obes Relat Metab Disord. 1999 Dec;23(12):1327-32.