PMID- 10642578
OWN - NLM
STAT- MEDLINE
DCOM- 20000225
LR  - 20190513
IS  - 0006-3363 (Print)
IS  - 0006-3363 (Linking)
VI  - 62
IP  - 2
DP  - 2000 Feb
TI  - Characterization of ovarian carbonyl reductase gene expression during ovulation
      in the gonadotropin-primed immature Rat.
PG  - 390-7
AB  - In this differential-display polymerase chain reaction-based study, four
      different primer sets generated cDNA fragments of ovarian carbonyl reductase
      genes that were uniquely expressed during the ovulatory process in eCG-primed
      immature rats. The temporal pattern of expression of this aldo-keto reductase
      gene was delineated by extracting ovarian RNA at 0, 2, 4, 8, 12, and 24 h after
      induction of ovulation via injection of the primed animals with hCG. The results 
      showed that at least four homologous forms of this gene were transcribed during
      ovulation. Northern blot analyses indicated a 14-fold increase in ovarian mRNA
      for carbonyl reductase, with expression reaching a peak at 8 h after hCG
      treatment and then declining to negligible levels during the next 16 h. In situ
      hybridization revealed that most of the transcription was in the thecal
      connective tissue of the ovary and was absent from the granulosa layer of ovarian
      follicles. Treatment of the animals with ovulation-blocking doses of epostane (an
      inhibitor of progesterone synthesis) or indomethacin (an inhibitor of prostanoid 
      synthesis) did not reduce the expression of ovarian carbonyl reductase.
      Nevertheless, the temporal pattern of expression of carbonyl reductase after the 
      induction of ovulation suggests that this enzyme activity is at least indirectly 
      associated with the ovulatory process.
FAU - Espey, L L
AU  - Espey LL
AD  - Department of Biology, Trinity University, San Antonio, Texas 78212. USA.
FAU - Yoshioka, S
AU  - Yoshioka S
FAU - Russell, D
AU  - Russell D
FAU - Ujioka, T
AU  - Ujioka T
FAU - Vladu, B
AU  - Vladu B
FAU - Skelsey, M
AU  - Skelsey M
FAU - Fujii, S
AU  - Fujii S
FAU - Okamura, H
AU  - Okamura H
FAU - Richards, J S
AU  - Richards JS
LA  - eng
GR  - HD16229/HD/NICHD NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Biol Reprod
JT  - Biology of reproduction
JID - 0207224
RN  - 0 (Androstenols)
RN  - 0 (Anti-Inflammatory Agents, Non-Steroidal)
RN  - 0 (DNA, Complementary)
RN  - 0 (Gonadotropins)
RN  - 0 (RNA, Messenger)
RN  - 6375T36951 (epostane)
RN  - EC 1.1.- (Alcohol Oxidoreductases)
RN  - EC 1.1.1.- (Aldo-Keto Reductases)
RN  - EC 1.1.1.21 (Aldehyde Reductase)
RN  - XXE1CET956 (Indomethacin)
SB  - IM
MH  - Alcohol Oxidoreductases/biosynthesis/*genetics
MH  - Aldehyde Reductase
MH  - Aldo-Keto Reductases
MH  - Androstenols/pharmacology
MH  - Animals
MH  - Anti-Inflammatory Agents, Non-Steroidal/pharmacology
MH  - Base Sequence
MH  - Blotting, Northern
MH  - DNA, Complementary/biosynthesis/genetics
MH  - Densitometry
MH  - Electrophoresis, Polyacrylamide Gel
MH  - Escherichia coli/metabolism
MH  - Female
MH  - Gene Expression Regulation, Developmental/*physiology
MH  - Gonadotropins/*pharmacology
MH  - In Situ Hybridization
MH  - Indomethacin/pharmacology
MH  - Male
MH  - Molecular Sequence Data
MH  - Ovary/cytology/*enzymology
MH  - Ovulation/*physiology
MH  - RNA, Messenger/biosynthesis/isolation & purification
MH  - Rats
MH  - Rats, Wistar
MH  - Reverse Transcriptase Polymerase Chain Reaction
EDAT- 2000/01/22 09:00
MHDA- 2000/03/04 09:00
CRDT- 2000/01/22 09:00
PHST- 2000/01/22 09:00 [pubmed]
PHST- 2000/03/04 09:00 [medline]
PHST- 2000/01/22 09:00 [entrez]
AID - 10.1095/biolreprod62.2.390 [doi]
PST - ppublish
SO  - Biol Reprod. 2000 Feb;62(2):390-7. doi: 10.1095/biolreprod62.2.390.