PMID- 10642556
OWN - NLM
STAT- MEDLINE
DCOM- 20000225
LR  - 20190513
IS  - 0006-3363 (Print)
IS  - 0006-3363 (Linking)
VI  - 62
IP  - 2
DP  - 2000 Feb
TI  - Mouse oviduct-specific glycoprotein gene: genomic organization and structure of
      the 5'-flanking regulatory region.
PG  - 217-26
AB  - A member of the chitinase protein family, oviduct-specific glycoprotein (OGP),
      can directly associate with gametes or with the early embryo in the oviduct.
      Although the glycoprotein is widely distributed among mammalian species and there
      is indirect evidence concerning the involvement of the molecule in the
      fertilization process, its physiological functions are far from completely
      understood. To understand the fundamental mechanisms that direct gene expression 
      as well as to know the physiological significance of OGP, we have isolated and
      characterized a mouse OGP gene (mogp-1). The gene was found to span 13.4
      kilobases (kb) including 11 exons and 10 introns. The genomic organization of
      mogp-1 is well conserved compared to the other members of the chitinase family.
      Two transcription initiation sites were found at positions 18 and 14 upstream
      from the first ATG codon. Fluorescence in situ hybridization analysis
      demonstrated that the mogp-1 was located on the R-positive F3 band of mouse
      chromosome 3. Although the putative promoter region of mogp-1 lacked typical
      TATA, CAAT, or GC box sequences, the region contained several motif sequences of 
      transcription factor binding sites including 10 half-palindromic estrogen
      responsive elements (ERE) and an imperfect ERE. Transient transfection
      experiments demonstrated that promoter activity could be modulated by various
      sequences within the 2.2 kb of the 5'-flanking region, and that the mogp-1
      promoter was transactivated in an estrogen receptor-positive cell line, MCF-7, by
      the addition of estradiol-17beta (E(2)). In addition, relevant promoter activity 
      for E(2) responsiveness resides within the first 270 base pairs upstream of the
      mogp-1. These findings should facilitate our understanding of the regulation of
      OGP gene expression, and they may be helpful for designing experiments to unravel
      the role of OGP in the process of mammalian fertilization.
FAU - Takahashi, K
AU  - Takahashi K
AD  - Department of Immunology & Parasitology and Obstetrics & Gynecology, Yamagata
      University School of Medicine, Research Institute for the Functional Peptides,
      Yamagata-City 990-9585, Japan.
FAU - Sendai, Y
AU  - Sendai Y
FAU - Matsuda, Y
AU  - Matsuda Y
FAU - Hoshi, H
AU  - Hoshi H
FAU - Hiroi, M
AU  - Hiroi M
FAU - Araki, Y
AU  - Araki Y
LA  - eng
SI  - GENBANK/AB006193
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Biol Reprod
JT  - Biology of reproduction
JID - 0207224
RN  - 0 (DNA Primers)
RN  - 0 (Glycoproteins)
RN  - 0 (Ovgp1 protein, mouse)
RN  - 0 (Receptors, Estrogen)
RN  - 9007-49-2 (DNA)
RN  - EC 1.13.12.- (Luciferases)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Cell Line
MH  - Chromosomes/genetics
MH  - Cloning, Molecular
MH  - DNA/*genetics
MH  - DNA Primers
MH  - Female
MH  - Gene Expression Regulation, Developmental/*genetics
MH  - Genes, Reporter/genetics
MH  - Genome
MH  - Glycoproteins/*genetics
MH  - Luciferases/genetics
MH  - Mice
MH  - Molecular Sequence Data
MH  - Plasmids
MH  - Receptors, Estrogen/genetics
MH  - Regulatory Sequences, Nucleic Acid/*genetics
MH  - Transfection/genetics
EDAT- 2000/01/22 09:00
MHDA- 2000/03/04 09:00
CRDT- 2000/01/22 09:00
PHST- 2000/01/22 09:00 [pubmed]
PHST- 2000/03/04 09:00 [medline]
PHST- 2000/01/22 09:00 [entrez]
AID - 10.1095/biolreprod62.2.217 [doi]
PST - ppublish
SO  - Biol Reprod. 2000 Feb;62(2):217-26. doi: 10.1095/biolreprod62.2.217.