PMID- 10642555
OWN - NLM
STAT- MEDLINE
DCOM- 20000207
LR  - 20190619
IS  - 0036-8075 (Print)
IS  - 0036-8075 (Linking)
VI  - 287
IP  - 5452
DP  - 2000 Jan 21
TI  - Rad6-dependent ubiquitination of histone H2B in yeast.
PG  - 501-4
AB  - Although ubiquitinated histones are present in substantial levels in vertebrate 
      cells, the roles they play in specific biological processes and the cellular 
      factors that regulate this modification are not well characterized. Ubiquitinated 
      H2B (uH2B) has been identified in the yeast Saccharomyces cerevisiae, and 
      mutation of the conserved ubiquitination site is shown to confer defects in 
      mitotic cell growth and meiosis. uH2B was not detected in rad6 mutants, which are 
      defective for the ubiquitin-conjugating enzyme Ubc2, thus identifying Rad6 as the 
      major cellular activity that ubiquitinates H2B in yeast.
FAU - Robzyk, K
AU  - Robzyk K
AD  - Program in Molecular Biology, Sloan-Kettering Cancer Center, 1275 York Avenue, 
      New York, NY 10021, USA.
FAU - Recht, J
AU  - Recht J
FAU - Osley, M A
AU  - Osley MA
LA  - eng
GR  - R01 GM040118/GM/NIGMS NIH HHS/United States
GR  - GM40118/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Science
JT  - Science (New York, N.Y.)
JID - 0404511
RN  - 0 (Histones)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 0 (Saccharomyces cerevisiae Proteins)
RN  - 0 (Ubiquitins)
RN  - EC 2.3.2.23 (RAD6 protein, S cerevisiae)
RN  - EC 2.3.2.23 (Ubiquitin-Conjugating Enzymes)
RN  - EC 6.- (Ligases)
SB  - IM
MH  - Amino Acid Substitution
MH  - Histones/*metabolism
MH  - Ligases/genetics/*metabolism
MH  - Meiosis
MH  - Mitosis
MH  - Mutagenesis, Site-Directed
MH  - Phenotype
MH  - Recombinant Fusion Proteins/metabolism
MH  - Saccharomyces cerevisiae/genetics/*metabolism/physiology
MH  - *Saccharomyces cerevisiae Proteins
MH  - Spores, Fungal/physiology
MH  - Substrate Specificity
MH  - Ubiquitin-Conjugating Enzymes
MH  - Ubiquitins/*metabolism
EDAT- 2000/01/22 00:00
MHDA- 2000/01/22 00:01
CRDT- 2000/01/22 00:00
PHST- 2000/01/22 00:00 [pubmed]
PHST- 2000/01/22 00:01 [medline]
PHST- 2000/01/22 00:00 [entrez]
AID - 8193 [pii]
AID - 10.1126/science.287.5452.501 [doi]
PST - ppublish
SO  - Science. 2000 Jan 21;287(5452):501-4. doi: 10.1126/science.287.5452.501.