PMID- 10642537
OWN - NLM
STAT- MEDLINE
DCOM- 20000323
LR  - 20181113
IS  - 0264-6021 (Print)
IS  - 0264-6021 (Linking)
VI  - 345 Pt 3
DP  - 2000 Feb 1
TI  - Characterization and gene structure of a novel retinoblastoma-protein-associated 
      protein similar to the transcription regulator TFII-I.
PG  - 749-57
AB  - Retinoblastoma protein (Rb) is an important regulator of vertebrate cell cycle
      and development. It functions through a direct interaction with protein factors
      involved in cell cycle progression and differentiation. In the present study we
      characterized a novel Rb-associated protein, Cream1, which bound to Rb
      specifically through a C-terminal region. Cream1 contained 959 amino acid
      residues and migrated as a protein of approx. 120 kDa on SDS/PAGE. It was a
      widely expressed nuclear protein with a nuclear localization signal resembling
      that of the large T antigen of simian virus 40. Its primary sequence was
      characteristic of five direct repeats that were similar to, but distinct from,
      those of TFII-I, a multifunctional transcription regulator. Three additional
      regions were also highly conserved in both proteins. Cream1 exhibited an
      activation activity that was attributed to its N-terminal portion when assayed in
      yeast. Its relationship with the muscle-enhancer-binding protein MusTRD1 further 
      suggests a role in regulating gene expression. The structural gene, CREAM1,
      contained 27 exons and spanned more than 150 kb. It was located at human
      chromosome 7q11.23 in a region deleted for Williams' syndrome, a
      neurodevelopmental disease with multisystem abnormalities, implying its
      involvement in certain disorders. Taken together, our results suggest that Cream1
      might serve as a positive transcription regulator under the control of Rb.
FAU - Yan, X
AU  - Yan X
AD  - Shanghai Research Center of Life Sciences, Chinese Academy of Sciences, 320 Yue
      Yang Road, Shanghai 200031, China.
FAU - Zhao, X
AU  - Zhao X
FAU - Qian, M
AU  - Qian M
FAU - Guo, N
AU  - Guo N
FAU - Gong, X
AU  - Gong X
FAU - Zhu, X
AU  - Zhu X
LA  - eng
SI  - GENBANK/AF089107
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Biochem J
JT  - The Biochemical journal
JID - 2984726R
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (GTF2IRD1 protein, human)
RN  - 0 (Muscle Proteins)
RN  - 0 (Nuclear Proteins)
RN  - 0 (Recombinant Proteins)
RN  - 0 (Retinoblastoma Protein)
RN  - 0 (Trans-Activators)
RN  - 0 (Transcription Factors)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - *Chromosomes, Human, Pair 7
MH  - Cloning, Molecular
MH  - DNA-Binding Proteins/genetics/metabolism
MH  - Humans
MH  - Molecular Sequence Data
MH  - *Muscle Proteins
MH  - Nuclear Proteins/*genetics/*metabolism
MH  - Recombinant Proteins/genetics/immunology/metabolism
MH  - Retinoblastoma Protein/*metabolism
MH  - Trans-Activators/*genetics/*metabolism
MH  - Transcription Factors/genetics/metabolism
MH  - Transcription, Genetic
MH  - Williams Syndrome/*genetics
PMC - PMC1220813
EDAT- 2000/01/22 09:00
MHDA- 2000/03/25 09:00
CRDT- 2000/01/22 09:00
PHST- 2000/01/22 09:00 [pubmed]
PHST- 2000/03/25 09:00 [medline]
PHST- 2000/01/22 09:00 [entrez]
PST - ppublish
SO  - Biochem J. 2000 Feb 1;345 Pt 3:749-57.