PMID- 10642536
OWN - NLM
STAT- MEDLINE
DCOM- 20000323
LR  - 20181113
IS  - 0264-6021 (Print)
IS  - 0264-6021 (Linking)
VI  - 345 Pt 3
DP  - 2000 Feb 1
TI  - 14-3-3zeta interacts with the alpha-chain of human interleukin 9 receptor.
PG  - 741-7
AB  - Interleukin 9 (IL-9) exerts its pleiotropic effects through the IL-9 receptor
      (IL-9R) complex, which consists of the IL-9R alpha-chain, which determines the
      cytokine specificity, and the IL-2 receptor gamma-chain. In the present study we 
      used a modified yeast two-hybrid system to isolate cDNA species encoding proteins
      that interacted with the intracellular domain of the human IL-9R alpha-chain
      (hIL-9Ralpha). We have identified 14-3-3zeta as an hIL-9Ralpha-interacting
      protein. We also mapped residues 518-522 (Arg-Ser(519)-Trp-Thr(521)-Phe) in
      hIL-9Ralpha and helix I of 14-3-3zeta as being important for interaction.
      Moreover, peptide competition experi-ments suggested that interaction between
      hIL-9Ralpha and 14-3-3zeta requires the phosphorylation of Ser(519) or Thr(521). 
      This is the first demonstration that 14-3-3 can interact with a non-tyrosine
      kinase receptor. The interaction between 14-3-3 and IL-9Ralpha but not IL-4Ralpha
      also suggests a potential role for 14-3-3 in determining cytokine specificity.
FAU - Sliva, D
AU  - Sliva D
AD  - Department of Medicine (Hematology/Oncology), Indiana University School of
      Medicine, and Indiana Cancer Research Institute, 1044 W. Walnut Street, R4-272,
      Indianapolis, IN 46202, USA.
FAU - Gu, M
AU  - Gu M
FAU - Zhu, Y X
AU  - Zhu YX
FAU - Chen, J
AU  - Chen J
FAU - Tsai, S
AU  - Tsai S
FAU - Du, X
AU  - Du X
FAU - Yang, Y C
AU  - Yang YC
LA  - eng
GR  - R01CA78433/CA/NCI NIH HHS/United States
GR  - R01DK50570/DK/NIDDK NIH HHS/United States
GR  - R01HL48819/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - Biochem J
JT  - The Biochemical journal
JID - 2984726R
RN  - 0 (14-3-3 Proteins)
RN  - 0 (IL9R protein, human)
RN  - 0 (Protein Isoforms)
RN  - 0 (Proteins)
RN  - 0 (Receptors, Interleukin)
RN  - 0 (Receptors, Interleukin-9)
RN  - 2ZD004190S (Threonine)
RN  - 452VLY9402 (Serine)
RN  - EC 1.14.16.2 (Tyrosine 3-Monooxygenase)
SB  - IM
MH  - 14-3-3 Proteins
MH  - Amino Acid Sequence
MH  - Binding Sites
MH  - Humans
MH  - Molecular Sequence Data
MH  - Phosphorylation
MH  - Precipitin Tests
MH  - Protein Isoforms/genetics/metabolism
MH  - Proteins/genetics/*metabolism
MH  - Receptors, Interleukin/genetics/*metabolism
MH  - Receptors, Interleukin-9
MH  - Serine/metabolism
MH  - Threonine/metabolism
MH  - Two-Hybrid System Techniques
MH  - *Tyrosine 3-Monooxygenase
PMC - PMC1220812
EDAT- 2000/01/22 09:00
MHDA- 2000/03/25 09:00
CRDT- 2000/01/22 09:00
PHST- 2000/01/22 09:00 [pubmed]
PHST- 2000/03/25 09:00 [medline]
PHST- 2000/01/22 09:00 [entrez]
PST - ppublish
SO  - Biochem J. 2000 Feb 1;345 Pt 3:741-7.