PMID- 10642534
OWN - NLM
STAT- MEDLINE
DCOM- 20000323
LR  - 20190501
IS  - 0264-6021 (Print)
IS  - 0264-6021 (Linking)
VI  - 345 Pt 3
DP  - 2000 Feb 1
TI  - Characterization of a second member of the subfamily of calcium-binding
      mitochondrial carriers expressed in human non-excitable tissues.
PG  - 725-32
AB  - We have recently identified a subfamily of mitochondrial carriers that bind
      calcium, and cloned ARALAR1, a member of this subfamily expressed in human muscle
      and brain. We have now cloned a second human ARALAR gene (ARALAR2) coding for a
      protein 78.3% identical to Aralar1, but expressed in liver and non-excitable
      tissues. Aralar2 is identical to citrin, the product of the gene mutated in
      type-II citrullinaemia [Kobayashi, Sinasac, Iijima, Boright, Begum, Lee, Yasuda, 
      Ikeda, Hirano, Terazono et al. (1999) Nat. Genet. 22, 159-163]. A related
      protein, DmAralar, 69% identical to Aralar1, was found in Drosophila
      melanogaster, the DMARALAR locus lying on the right arm of the third chromosome, 
      band 99F. The N-terminal half of Aralar2/citrin is able to bind calcium and this 
      requires the presence of the two most distal EF-hands. The localization of
      Aralar2/citrin expressed in human cell lines is mitochondrial, the C-terminal
      half containing sufficient information for import and assembly into mitochondria.
      The C-terminal half of Aralar proteins is related to the yeast YPR020c gene, with
      a very high sequence conservation (54.3% identity), suggesting that these
      proteins play an important role. Thus Aralar proteins are probably expressed in
      all tissues in an isoform-specific fashion, where they function as
      calcium-regulated metabolite (possibly anionic) carriers.
FAU - Del Arco, A
AU  - Del Arco A
AD  - Departamento de Biologia Molecular, Centro de Biologia Molecular 'Severo Ochoa', 
      Campus de Cantoblanco, 28049-Madrid, Spain.
FAU - Agudo, M
AU  - Agudo M
FAU - Satrustegui, J
AU  - Satrustegui J
LA  - eng
SI  - GENBANK/A99865
SI  - GENBANK/AA360112
SI  - GENBANK/AA410516
SI  - GENBANK/AA511635
SI  - GENBANK/H59215
SI  - GENBANK/T55265
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Biochem J
JT  - The Biochemical journal
JID - 2984726R
RN  - 0 (Aralar protein, Drosophila)
RN  - 0 (Calcium-Binding Proteins)
RN  - 0 (Drosophila Proteins)
RN  - 0 (Insect Proteins)
RN  - 0 (Membrane Transport Proteins)
RN  - 0 (Mitochondrial Membrane Transport Proteins)
RN  - 0 (Mitochondrial Proteins)
RN  - 0 (Organic Anion Transporters)
RN  - 0 (SLC25A12 protein, human)
RN  - 1340-08-5 (citrin)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Blotting, Northern
MH  - Calcium-Binding Proteins/*genetics/*metabolism
MH  - Child, Preschool
MH  - Cloning, Molecular
MH  - Drosophila/genetics
MH  - *Drosophila Proteins
MH  - Humans
MH  - Insect Proteins/*genetics/*metabolism
MH  - Liver/metabolism
MH  - *Membrane Transport Proteins
MH  - Mitochondria/*metabolism
MH  - Mitochondrial Membrane Transport Proteins
MH  - *Mitochondrial Proteins
MH  - Molecular Sequence Data
MH  - *Organic Anion Transporters
MH  - Sequence Homology, Amino Acid
PMC - PMC1220810
EDAT- 2000/01/22 09:00
MHDA- 2000/03/25 09:00
CRDT- 2000/01/22 09:00
PHST- 2000/01/22 09:00 [pubmed]
PHST- 2000/03/25 09:00 [medline]
PHST- 2000/01/22 09:00 [entrez]
AID - 10.1042/0264-6021:3450725 [doi]
PST - ppublish
SO  - Biochem J. 2000 Feb 1;345 Pt 3:725-32. doi: 10.1042/0264-6021:3450725.