PMID- 10642496
OWN - NLM
STAT- MEDLINE
DCOM- 20000323
LR  - 20181113
IS  - 0264-6021 (Print)
IS  - 0264-6021 (Linking)
VI  - 345 Pt 3
DP  - 2000 Feb 1
TI  - Cytoplasmic STAT proteins associate prior to activation.
PG  - 417-21
AB  - The commonly accepted model of STAT factor activation at the cytoplasmic part of 
      the receptor assumes that signal transducers and activators of transcription
      (STATs) are recruited from a cytoplasmic pool of monomeric STAT proteins. Based
      on a previous observation that non-phosphorylated STAT3-Src homology 2 domains
      dimerize in vitro, we investigated whether the observed dimerization is of
      physiological relevance within the cellular context. We show that STAT1 and STAT3
      are pre-associated in non-stimulated cells. Apparently, these complexes are not
      able to translocate into the nucleus. We provide evidence that the event of STAT 
      activation is more complex than previously assumed.
FAU - Haan, S
AU  - Haan S
AD  - Institut fur Biochemie, Rheinisch-Westfalische Technische Hochschule Aachen,
      Pauwelsstrasse 30, D-52074 Aachen, Germany.
FAU - Kortylewski, M
AU  - Kortylewski M
FAU - Behrmann, I
AU  - Behrmann I
FAU - Muller-Esterl, W
AU  - Muller-Esterl W
FAU - Heinrich, P C
AU  - Heinrich PC
FAU - Schaper, F
AU  - Schaper F
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Biochem J
JT  - The Biochemical journal
JID - 2984726R
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Interleukin-6)
RN  - 0 (STAT1 Transcription Factor)
RN  - 0 (STAT1 protein, human)
RN  - 0 (STAT3 Transcription Factor)
RN  - 0 (STAT3 protein, human)
RN  - 0 (Trans-Activators)
RN  - 42HK56048U (Tyrosine)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - COS Cells/metabolism
MH  - Carcinoma, Hepatocellular/drug therapy/metabolism
MH  - Cross Reactions
MH  - Cytoplasm/drug effects/*metabolism
MH  - DNA-Binding Proteins/drug effects/immunology/*metabolism
MH  - Dimerization
MH  - Humans
MH  - Interleukin-6/metabolism/pharmacology
MH  - Liver Neoplasms/drug therapy/metabolism
MH  - Melanoma/drug therapy/metabolism
MH  - Molecular Sequence Data
MH  - Phosphorylation
MH  - Precipitin Tests
MH  - STAT1 Transcription Factor
MH  - STAT3 Transcription Factor
MH  - Trans-Activators/drug effects/immunology/*metabolism
MH  - Transfection
MH  - Tumor Cells, Cultured
MH  - Tyrosine/metabolism
PMC - PMC1220772
EDAT- 2000/01/22 09:00
MHDA- 2000/03/25 09:00
CRDT- 2000/01/22 09:00
PHST- 2000/01/22 09:00 [pubmed]
PHST- 2000/03/25 09:00 [medline]
PHST- 2000/01/22 09:00 [entrez]
PST - ppublish
SO  - Biochem J. 2000 Feb 1;345 Pt 3:417-21.