PMID- 10640736
OWN - NLM
STAT- MEDLINE
DCOM- 20000224
LR  - 20190515
IS  - 0022-1767 (Print)
IS  - 0022-1767 (Linking)
VI  - 164
IP  - 3
DP  - 2000 Feb 1
TI  - Phosphorylation of FADD/ MORT1 at serine 194 and association with a 70-kDa cell
      cycle-regulated protein kinase.
PG  - 1236-42
AB  - The adapter molecule Fas-associated death domain protein (FADD)/mediator of
      receptor-induced toxicity-1 (MORT1) is essential for signal transduction of the
      apoptosis-inducing receptor CD95 (APO-1/Fas) as it connects the activated
      receptor with the effector caspase-8. FADD also plays a role in embryonic
      development and the cell cycle reentry of T cells. FADD is phosphorylated at
      serine residues. We now show that phosphorylation exclusively occurs at serine
      194. The phosphorylation of FADD was found to correlate with the cell cycle. In
      cells arrested at the G2/M boundary with nocodazole, FADD was quantitatively
      phosphorylated, whereas only nonphosphorylated FADD was found in cells arrested
      in G1/S with hydroxyurea. In this context, we have identified a 70-kDa cell
      cycle-regulated kinase that specifically binds to the C-terminal half of FADD.
      Because CD95-mediated apoptosis is independent of the cell cycle, phosphorylation
      of FADD may regulate its apoptosis-independent functions.
FAU - Scaffidi, C
AU  - Scaffidi C
AD  - Tumor Immunology Program, Applied Tumorvirology, German Cancer Research Center,
      Heidelberg, Germany.
FAU - Volkland, J
AU  - Volkland J
FAU - Blomberg, I
AU  - Blomberg I
FAU - Hoffmann, I
AU  - Hoffmann I
FAU - Krammer, P H
AU  - Krammer PH
FAU - Peter, M E
AU  - Peter ME
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Immunol
JT  - Journal of immunology (Baltimore, Md. : 1950)
JID - 2985117R
RN  - 0 (Adaptor Proteins, Signal Transducing)
RN  - 0 (Carrier Proteins)
RN  - 0 (FADD protein, human)
RN  - 0 (Fas-Associated Death Domain Protein)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 0 (fas Receptor)
RN  - 452VLY9402 (Serine)
RN  - EC 2.7.11.22 (Cyclin-Dependent Kinases)
SB  - AIM
SB  - IM
MH  - *Adaptor Proteins, Signal Transducing
MH  - Burkitt Lymphoma
MH  - Carrier Proteins/genetics/*metabolism
MH  - Cell Cycle/immunology
MH  - Cell Line
MH  - Cyclin-Dependent Kinases/*metabolism
MH  - Cytotoxicity, Immunologic/genetics
MH  - Fas-Associated Death Domain Protein
MH  - Genetic Vectors/chemical synthesis/metabolism
MH  - Humans
MH  - Molecular Weight
MH  - Phosphorylation
MH  - Recombinant Fusion Proteins/metabolism
MH  - Serine/*metabolism
MH  - Transfection
MH  - Tumor Cells, Cultured
MH  - fas Receptor/genetics/*metabolism
EDAT- 2000/01/21 09:00
MHDA- 2000/02/26 09:00
CRDT- 2000/01/21 09:00
PHST- 2000/01/21 09:00 [pubmed]
PHST- 2000/02/26 09:00 [medline]
PHST- 2000/01/21 09:00 [entrez]
AID - ji_v164n3p1236 [pii]
AID - 10.4049/jimmunol.164.3.1236 [doi]
PST - ppublish
SO  - J Immunol. 2000 Feb 1;164(3):1236-42. doi: 10.4049/jimmunol.164.3.1236.