PMID- 10640688
OWN - NLM
STAT- MEDLINE
DCOM- 20000215
LR  - 20191210
IS  - 0169-328X (Print)
IS  - 0169-328X (Linking)
VI  - 74
IP  - 1-2
DP  - 1999 Dec 10
TI  - Discovery of a putative heme-binding protein family (SOUL/HBP) by two-tissue
      suppression subtractive hybridization and database searches.
PG  - 175-81
AB  - In the domestic chicken, Gallus gallus, the retina and pineal gland contain
      circadian clocks that are directly entrained by environmental light-dark cycles. 
      To identify novel genes that are expressed in the retina and pineal gland, we
      performed two-tissue suppression subtractive hybridization (SSH). Two-tissue SSH 
      is designed to identify genes expressed in common between two RNA samples while
      at the same time subtracting out abundant transcripts. Using this method, we
      identified a novel chicken gene, named ckSoul, that is strongly expressed in the 
      retina and pineal gland. The protein product of ckSoul is similar to a novel
      heme-binding protein (p22 HBP) and to an uncharacterized mammalian gene in the
      expressed sequence tag (EST) database. The mouse transcript of this new gene is
      expressed in the retina and may represent the mammalian ortholog of ckSoul.
      Molecular analysis of the mammalian and chicken proteins suggests SOUL and HBP
      are members of a new family of heme-binding proteins.
FAU - Zylka, M J
AU  - Zylka MJ
AD  - Laboratory of Developmental Chronobiology, Pediatric Service, Massachusetts
      General Hospital, and Harvard Medical School, Boston, MA 02114, USA.
FAU - Reppert, S M
AU  - Reppert SM
LA  - eng
SI  - GENBANK/AF117612
SI  - GENBANK/AF117613
SI  - GENBANK/AF117614
SI  - GENBANK/AF117615
SI  - GENBANK/AF117616
SI  - GENBANK/AI438107
SI  - GENBANK/AI438108
SI  - GENBANK/AI438109
SI  - GENBANK/AI438110
SI  - GENBANK/AI438111
SI  - GENBANK/AI438112
SI  - GENBANK/AI438113
SI  - GENBANK/AI438114
SI  - GENBANK/AI438115
SI  - GENBANK/AI438116
SI  - GENBANK/AI438117
SI  - GENBANK/AI438118
SI  - GENBANK/AI438119
SI  - GENBANK/AI438120
SI  - GENBANK/AI438121
SI  - GENBANK/AI438122
SI  - GENBANK/AI438123
SI  - GENBANK/AI438124
SI  - GENBANK/AI438125
SI  - GENBANK/AI438126
SI  - GENBANK/AI438127
SI  - GENBANK/AI438128
SI  - GENBANK/AI438129
SI  - GENBANK/AI438130
SI  - GENBANK/AI438131
SI  - etc.
GR  - GM55820/GM/NIGMS NIH HHS/United States
GR  - MH11547/MH/NIMH NIH HHS/United States
GR  - R37HD14427/HD/NICHD NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Netherlands
TA  - Brain Res Mol Brain Res
JT  - Brain research. Molecular brain research
JID - 8908640
RN  - 0 (Carrier Proteins)
RN  - 0 (DNA, Complementary)
RN  - 0 (HEBP2 protein, human)
RN  - 0 (Heme-Binding Proteins)
RN  - 0 (Hemeproteins)
RN  - 0 (Pregnancy Proteins)
RN  - 63231-63-0 (RNA)
SB  - IM
MH  - 3T3 Cells
MH  - Amino Acid Sequence
MH  - Animals
MH  - Blotting, Northern
MH  - Carrier Proteins/*genetics
MH  - Chickens
MH  - Cloning, Molecular
MH  - DNA, Complementary/chemistry/genetics
MH  - *Databases, Factual
MH  - Female
MH  - Gene Expression
MH  - Heme-Binding Proteins
MH  - Hemeproteins/*genetics
MH  - Humans
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Inbred Strains
MH  - Molecular Sequence Data
MH  - Nucleic Acid Hybridization
MH  - Pineal Gland/metabolism
MH  - Pregnancy Proteins/*genetics
MH  - RNA/genetics/metabolism
MH  - Retina/metabolism
MH  - Sequence Alignment
MH  - Sequence Analysis, DNA
MH  - Sequence Homology, Amino Acid
MH  - Tissue Distribution
EDAT- 2000/01/21 09:00
MHDA- 2000/02/19 09:00
CRDT- 2000/01/21 09:00
PHST- 2000/01/21 09:00 [pubmed]
PHST- 2000/02/19 09:00 [medline]
PHST- 2000/01/21 09:00 [entrez]
AID - S0169328X99002776 [pii]
AID - 10.1016/s0169-328x(99)00277-6 [doi]
PST - ppublish
SO  - Brain Res Mol Brain Res. 1999 Dec 10;74(1-2):175-81. doi:
      10.1016/s0169-328x(99)00277-6.