PMID- 10640683
OWN - NLM
STAT- MEDLINE
DCOM- 20000215
LR  - 20190826
IS  - 0169-328X (Print)
IS  - 0169-328X (Linking)
VI  - 74
IP  - 1-2
DP  - 1999 Dec 10
TI  - Interaction of PKN with a neuron-specific basic helix-loop-helix transcription
      factor, NDRF/NeuroD2.
PG  - 126-34
AB  - By the yeast two-hybrid screening of a human brain cDNA library with the
      amino-terminal regulatory region of PKN as a bait, a clone encoding a
      neuron-specific basic Helix-Loop-Helix (bHLH) transcription factor, NDRF/NeuroD2 
      was isolated. NDRF/NeuroD2 was co-precipitated with PKN from the lysate of COS-7 
      cells transfected with both expression constructs for NDRF/NeuroD2 and PKN. In
      vitro binding studies using the deletion mutants of NDRF/NeuroD2 synthesized in a
      rabbit reticulocyte lysate indicated that the internal region containing the bHLH
      domain of NDRF/NeuroD2 was necessary and sufficient for the interaction with PKN.
      In addition, recombinant NDRF/NeuroD2 purified from Escherichia coli could bind
      PKN, suggesting the direct interaction between NDRF/NeuroD2 and PKN. Transient
      transfection assays using P19 cells revealed that expression of NDRF/NeuroD2
      increased the transactivation of the rat insulin promoter element 3 (RIPE3)
      enhancer up to approximately 12-fold and that co-expression of catalytically
      active form of PKN, but not kinase-deficient derivative, resulted in a further
      threefold increase of NDRF/NeuroD2-mediated transcription. These findings suggest
      that PKN may contribute to transcriptional responses through the
      post-translational modification of the NDRF/NeuroD2-dependent transcriptional
      machinery.
FAU - Shibata, H
AU  - Shibata H
AD  - Department of Biology, Faculty of Science, Kobe University, Kobe, Japan.
FAU - Oda, H
AU  - Oda H
FAU - Mukai, H
AU  - Mukai H
FAU - Oishi, K
AU  - Oishi K
FAU - Misaki, K
AU  - Misaki K
FAU - Ohkubo, H
AU  - Ohkubo H
FAU - Ono, Y
AU  - Ono Y
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Brain Res Mol Brain Res
JT  - Brain research. Molecular brain research
JID - 8908640
RN  - 0 (Basic Helix-Loop-Helix Transcription Factors)
RN  - 0 (NEUROD2 protein, human)
RN  - 0 (Neurod2 protein, rat)
RN  - 0 (Neuropeptides)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 0 (Transcription Factors)
RN  - EC 2.7.1.- (protein kinase N)
RN  - EC 2.7.10.1 (Protein-Tyrosine Kinases)
RN  - EC 2.7.11.1 (Protein-Serine-Threonine Kinases)
RN  - EC 2.7.11.13 (Protein Kinase C)
SB  - IM
MH  - Animals
MH  - Basic Helix-Loop-Helix Transcription Factors
MH  - COS Cells
MH  - Catalysis
MH  - Cell Line
MH  - Gene Expression Regulation
MH  - *Helix-Loop-Helix Motifs
MH  - Humans
MH  - Mutation
MH  - Neuropeptides/genetics/isolation & purification/*metabolism
MH  - Protein Binding
MH  - Protein Kinase C
MH  - Protein-Serine-Threonine Kinases/genetics/*metabolism
MH  - Protein-Tyrosine Kinases/genetics/*metabolism
MH  - Rabbits
MH  - Rats
MH  - Recombinant Fusion Proteins/genetics/isolation & purification/metabolism
MH  - Transcription Factors/genetics/isolation & purification/*metabolism
MH  - Transcription, Genetic
MH  - Tumor Cells, Cultured
MH  - Two-Hybrid System Techniques
EDAT- 2000/01/21 09:00
MHDA- 2000/02/19 09:00
CRDT- 2000/01/21 09:00
PHST- 2000/01/21 09:00 [pubmed]
PHST- 2000/02/19 09:00 [medline]
PHST- 2000/01/21 09:00 [entrez]
AID - S0169328X99002739 [pii]
AID - 10.1016/s0169-328x(99)00273-9 [doi]
PST - ppublish
SO  - Brain Res Mol Brain Res. 1999 Dec 10;74(1-2):126-34. doi:
      10.1016/s0169-328x(99)00273-9.