PMID- 10640539
OWN - NLM
STAT- MEDLINE
DCOM- 20000223
LR  - 20111117
IS  - 0022-1317 (Print)
IS  - 0022-1317 (Linking)
VI  - 81
IP  - Pt 1
DP  - 2000 Jan
TI  - Cloning and epitope mapping of a functional partial fusion receptor for human
      cytomegalovirus gH.
PG  - 27-35
AB  - A cDNA clone encoding a partial putative human cytomegalovirus (HCMV) gH fusion
      receptor (CMVFR) was previously identified. In this report, the cDNA sequence of 
      CMVFR was determined and the role of this CMVFR in HCMV/cell fusion was confirmed
      by rendering fusion-incompetent MOLT-4 cells susceptible to fusion following
      transfection with receptor cDNA. Blocking experiments using recombinant gH or
      either of two MAbs (against recombinant gH or purified viral gH:gL) provided
      additional evidence for the role of gH binding to this protein in virus fusion.
      An HCMV-binding domain of 12 aa in the middle hydrophilic region of CMVFR was
      identified by fusion blocking studies using synthetic receptor peptides. The 1368
      bp cDNA of CMVFR contained a predicted ORF of 345 aa with two potential
      membrane-spanning domains and several possible nuclear localization signals. A
      search of sequence databases indicated that CMVFR is a novel protein. Further
      characterization of this cell membrane protein that confers susceptibility to
      fusion with the viral envelope should provide important information about the
      mechanism by which HCMV infects cells.
FAU - Baldwin, B R
AU  - Baldwin BR
AD  - Molecular and Cell Biology Program, University of Maryland School of Medicine,
      Baltimore, MD 21201, USA. bbald001@umaryland.edu
FAU - Zhang, C O
AU  - Zhang CO
FAU - Keay, S
AU  - Keay S
LA  - eng
SI  - GENBANK/AF189251
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - England
TA  - J Gen Virol
JT  - The Journal of general virology
JID - 0077340
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (DNA, Complementary)
RN  - 0 (Receptors, Virus)
RN  - 0 (Viral Envelope Proteins)
RN  - 0 (glycoprotein H, Cytomegalovirus)
RN  - 0 (glycoprotein H, Human cytomegalovirus)
SB  - IM
MH  - Amino Acid Sequence
MH  - Antibodies, Monoclonal/immunology
MH  - Base Sequence
MH  - *Cloning, Molecular
MH  - Cytomegalovirus/genetics/immunology/metabolism/*pathogenicity
MH  - DNA, Complementary
MH  - *Epitope Mapping
MH  - Humans
MH  - Membrane Fusion
MH  - Molecular Sequence Data
MH  - Receptors, Virus/genetics/immunology/*metabolism
MH  - Sequence Analysis, DNA
MH  - Transfection
MH  - Tumor Cells, Cultured
MH  - Viral Envelope Proteins/*metabolism
MH  - Viral Plaque Assay
EDAT- 2000/01/21 09:00
MHDA- 2000/02/26 09:00
CRDT- 2000/01/21 09:00
PHST- 2000/01/21 09:00 [pubmed]
PHST- 2000/02/26 09:00 [medline]
PHST- 2000/01/21 09:00 [entrez]
AID - 10.1099/0022-1317-81-1-27 [doi]
PST - ppublish
SO  - J Gen Virol. 2000 Jan;81(Pt 1):27-35. doi: 10.1099/0022-1317-81-1-27.