PMID- 10640276
OWN - NLM
STAT- MEDLINE
DCOM- 20000215
LR  - 20181113
IS  - 0890-9369 (Print)
IS  - 0890-9369 (Linking)
VI  - 14
IP  - 1
DP  - 2000 Jan 1
TI  - Isolation of a novel histone deacetylase reveals that class I and class II
      deacetylases promote SMRT-mediated repression.
PG  - 55-66
AB  - The transcriptional corepressor SMRT functions by mediating the repressive effect
      of transcription factors involved in diverse signaling pathways. The mechanism by
      which SMRT represses basal transcription has been proposed to involve the
      indirect recruitment of histone deacetylase HDAC1 via the adaptor mSin3A. In
      contrast to this model, a two-hybrid screen on SMRT-interacting proteins resulted
      in the isolation of the recently described HDAC5 and a new family member termed
      HDAC7. Molecular and biochemical results indicate that this interaction is direct
      and in vivo evidence colocalizes SMRT, mHDAC5, and mHDAC7 to a distinct nuclear
      compartment. Surprisingly, HDAC7 can interact with mSin3A in yeast and in
      mammalian cells, suggesting association of multiple repression complexes. Taken
      together, our results provide the first evidence that SMRT-mediated repression is
      promoted by class I and class II histone deacetylases and that SMRT can recruit
      class II histone deacetylases in a mSin3A-independent fashion.
FAU - Kao, H Y
AU  - Kao HY
AD  - Howard Hughes Medical Institute, Gene Expression Laboratory, The Salk Institute
      for Biological Studies, La Jolla, California 92037 USA.
FAU - Downes, M
AU  - Downes M
FAU - Ordentlich, P
AU  - Ordentlich P
FAU - Evans, R M
AU  - Evans RM
LA  - eng
GR  - GM 26444/GM/NIGMS NIH HHS/United States
GR  - R01 HD027183/HD/NICHD NIH HHS/United States
GR  - HD27183/HD/NICHD NIH HHS/United States
GR  - CA54418/CA/NCI NIH HHS/United States
GR  - P01 CA054418/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Genes Dev
JT  - Genes & development
JID - 8711660
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Nuclear Receptor Co-Repressor 2)
RN  - 0 (Repressor Proteins)
RN  - EC 3.5.1.98 (Histone Deacetylases)
SB  - IM
MH  - Amino Acid Sequence
MH  - Cell Nucleus/metabolism
MH  - DNA-Binding Proteins/metabolism/*physiology
MH  - Histone Deacetylases/chemistry/*isolation & purification/metabolism
MH  - Molecular Sequence Data
MH  - Nuclear Receptor Co-Repressor 2
MH  - Repressor Proteins/metabolism/*physiology
MH  - Saccharomyces cerevisiae/enzymology
MH  - Sequence Homology, Amino Acid
MH  - Transcription, Genetic
PMC - PMC316336
EDAT- 2000/01/20 09:00
MHDA- 2000/02/19 09:00
CRDT- 2000/01/20 09:00
PHST- 2000/01/20 09:00 [pubmed]
PHST- 2000/02/19 09:00 [medline]
PHST- 2000/01/20 09:00 [entrez]
PST - ppublish
SO  - Genes Dev. 2000 Jan 1;14(1):55-66.