PMID- 10639434
OWN - NLM
STAT- MEDLINE
DCOM- 20000210
LR  - 20190508
IS  - 0019-9567 (Print)
IS  - 0019-9567 (Linking)
VI  - 68
IP  - 2
DP  - 2000 Feb
TI  - Mannose-binding lectin binds to a range of clinically relevant microorganisms and
      promotes complement deposition.
PG  - 688-93
AB  - Mannose-binding lectin (MBL) is a collagenous serum lectin believed to be of
      importance in innate immunity. Genetically determined low levels of the protein
      are known to predispose to infections. In this study the binding of purified MBL 
      to pathogens isolated from immunocompromised children was investigated by flow
      cytometry. Diverse Candida species, Aspergillus fumigatus, Staphylococcus aureus,
      and beta-hemolytic group A streptococci exhibited strong binding of MBL, whereas 
      Escherichia coli, Klebsiella species, and Haemophilus influenzae type b were
      characterized by heterogeneous binding patterns. In contrast, beta-hemolytic
      group B streptococci, Streptococcus pneumoniae, and Staphylococcus epidermidis
      showed low levels of binding. Bound MBL was able to promote C4 deposition in a
      concentration-dependent manner. We conclude that MBL may be of importance in
      first-line immune defense against several important pathogens.
FAU - Neth, O
AU  - Neth O
AD  - Immunobiology Unit, Institute of Child Health, University College London, NHS
      Trust, London, United Kingdom.
FAU - Jack, D L
AU  - Jack DL
FAU - Dodds, A W
AU  - Dodds AW
FAU - Holzel, H
AU  - Holzel H
FAU - Klein, N J
AU  - Klein NJ
FAU - Turner, M W
AU  - Turner MW
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Infect Immun
JT  - Infection and immunity
JID - 0246127
RN  - 0 (Carrier Proteins)
RN  - 0 (Collectins)
RN  - 0 (Complement C4)
SB  - IM
MH  - Aspergillus fumigatus/metabolism
MH  - Bacteria/metabolism
MH  - Candida/metabolism
MH  - Carrier Proteins/*metabolism/pharmacology
MH  - Child
MH  - Collectins
MH  - Complement C4/*metabolism
MH  - Dose-Response Relationship, Drug
MH  - Flow Cytometry
MH  - Humans
PMC - PMC97193
EDAT- 2000/01/20 00:00
MHDA- 2000/01/20 00:01
CRDT- 2000/01/20 00:00
PHST- 2000/01/20 00:00 [pubmed]
PHST- 2000/01/20 00:01 [medline]
PHST- 2000/01/20 00:00 [entrez]
AID - 10.1128/iai.68.2.688-693.2000 [doi]
PST - ppublish
SO  - Infect Immun. 2000 Feb;68(2):688-93. doi: 10.1128/iai.68.2.688-693.2000.