PMID- 10637769
OWN - NLM
STAT- MEDLINE
DCOM- 20000302
LR  - 20131121
IS  - 1521-6543 (Print)
IS  - 1521-6543 (Linking)
VI  - 48
IP  - 5
DP  - 1999 Nov
TI  - Phosphoserine aminotransferase, the second step-catalyzing enzyme for serine
      biosynthesis.
PG  - 525-9
AB  - As a step toward analyzing the serine biosynthetic pathway in mammals, we have
      studied the properties of phosphoserine aminotransferase, the second
      step-catalyzing enzyme. The K(m) values for 3-phosphohydroxypyruvate and
      L-phosphoserine are 5 and 35 microM, respectively, and those for glutamate and
      alpha-ketoglutarate are 1.2 and 0.8 mM, respectively. The product inhibition
      studies strengthened the support for a ping-pong mechanism and allowed evaluation
      of Ki values for the four substrates. The equilibrium constant evaluated from the
      kinetic parameters is approximately 40. Additionally, some physical properties
      relative to the bound coenzyme and the secondary structure were investigated. The
      results are consistent with a structural relationship between the Escherichia
      coli enzyme and the mammalian enzyme. The mammalian enzyme has specific kinetic
      parameters, the determination of which is a prerequisite to analyzing the serine 
      biosynthetic pathway in mammals.
FAU - Basurko, M J
AU  - Basurko MJ
AD  - Departement de Biochimie Medicale et Biologie Moleculaire, Universite Victor
      Segalen, Bordeaux, France.
FAU - Marche, M
AU  - Marche M
FAU - Darriet, M
AU  - Darriet M
FAU - Cassaigne, A
AU  - Cassaigne A
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - England
TA  - IUBMB Life
JT  - IUBMB life
JID - 100888706
RN  - 0 (Coenzymes)
RN  - 0 (Multienzyme Complexes)
RN  - 0 (Pyruvates)
RN  - 3913-50-6 (phosphohydroxypyruvic acid)
RN  - 452VLY9402 (Serine)
RN  - EC 2.6.1.- (Transaminases)
RN  - EC 2.6.1.52 (phosphoserine aminotransferase)
SB  - IM
MH  - Animals
MH  - Catalysis
MH  - Cattle
MH  - Coenzymes/metabolism
MH  - Kinetics
MH  - Multienzyme Complexes/antagonists & inhibitors/metabolism
MH  - Protein Structure, Secondary
MH  - Pyruvates/metabolism
MH  - Serine/*biosynthesis
MH  - Transaminases/antagonists & inhibitors/chemistry/*metabolism
EDAT- 2000/01/19 09:00
MHDA- 2000/03/04 09:00
CRDT- 2000/01/19 09:00
PHST- 2000/01/19 09:00 [pubmed]
PHST- 2000/03/04 09:00 [medline]
PHST- 2000/01/19 09:00 [entrez]
AID - 10.1080/713803557 [doi]
PST - ppublish
SO  - IUBMB Life. 1999 Nov;48(5):525-9. doi: 10.1080/713803557.