PMID- 10637334
OWN - NLM
STAT- MEDLINE
DCOM- 20000306
LR  - 20190501
IS  - 1362-4962 (Electronic)
IS  - 0305-1048 (Linking)
VI  - 28
IP  - 3
DP  - 2000 Feb 1
TI  - Isolation and characterization of human orthologs of yeast CCR4-NOT complex
      subunits.
PG  - 809-17
AB  - The yeast CCR4-NOT protein complex is a global regulator of RNA polymerase II
      transcription. It is comprised of yeast NOT1 to NOT5, yeast CCR4 and additional
      proteins like yeast CAF1. Here we report the isolation of cDNAs encoding human
      NOT2, NOT3, NOT4 and a CAF1-like factor, CALIF. Analysis of their mRNA levels in 
      different human tissues reveals a common ubiquitous expression pattern. A
      multitude of two-hybrid interactions among the human cDNAs suggest that their
      encoded proteins also form a complex in mammalian cells. Functional conservation 
      of these proteins throughout evolution is supported by the observation that the
      isolated human NOT3 and NOT4 cDNAs can partially com-plement corresponding not
      mutations in yeast. Interestingly, human CALIF is highly homologous to, although 
      clearly different from, a recently described human CAF1 protein. Conserved
      interactions of this factor with both NOT and CCR4 proteins and
      co-immunoprecipitation experiments suggest that CALIF is a bona fide component of
      the human CCR4-NOT complex.
FAU - Albert, T K
AU  - Albert TK
AD  - Laboratory for Physiological Chemistry and Centre for Biomedical Genetics,
      Utrecht University, PO Box 80042, 3508 TA Utrecht, The Netherlands,
FAU - Lemaire, M
AU  - Lemaire M
FAU - van Berkum, N L
AU  - van Berkum NL
FAU - Gentz, R
AU  - Gentz R
FAU - Collart, M A
AU  - Collart MA
FAU - Timmers, H T
AU  - Timmers HT
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Nucleic Acids Res
JT  - Nucleic acids research
JID - 0411011
RN  - 0 (CCR4 protein, human)
RN  - 0 (CDC36 protein, S cerevisiae)
RN  - 0 (CNOT8 protein, human)
RN  - 0 (Cell Cycle Proteins)
RN  - 0 (Fungal Proteins)
RN  - 0 (Proteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, CCR4)
RN  - 0 (Receptors, Chemokine)
RN  - 0 (Repressor Proteins)
RN  - 0 (Saccharomyces cerevisiae Proteins)
RN  - 0 (Transcription Factors)
RN  - EC 3.1.- (CCR4 protein, S cerevisiae)
RN  - EC 3.1.- (Ribonucleases)
RN  - EC 3.1.13.4 (POP2 protein, S cerevisiae)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Cell Cycle Proteins/chemistry/genetics/*metabolism
MH  - Cell Line
MH  - Cloning, Molecular
MH  - Conserved Sequence/*genetics
MH  - Expressed Sequence Tags
MH  - Fungal Proteins/genetics/*metabolism
MH  - Gene Expression Profiling
MH  - Genes, Fungal/genetics
MH  - Genetic Complementation Test
MH  - Humans
MH  - Molecular Sequence Data
MH  - Mutation/genetics
MH  - Precipitin Tests
MH  - Protein Binding
MH  - *Proteins
MH  - RNA, Messenger/analysis/genetics
MH  - Receptors, CCR4
MH  - Receptors, Chemokine
MH  - Repressor Proteins/chemistry/genetics/*metabolism
MH  - *Ribonucleases
MH  - Saccharomyces cerevisiae/chemistry/cytology/genetics/metabolism
MH  - *Saccharomyces cerevisiae Proteins
MH  - Transcription Factors/chemistry/genetics/*metabolism
MH  - Two-Hybrid System Techniques
PMC - PMC102560
EDAT- 2000/01/19 00:00
MHDA- 2000/03/11 00:00
CRDT- 2000/01/19 00:00
PHST- 2000/01/19 00:00 [pubmed]
PHST- 2000/03/11 00:00 [medline]
PHST- 2000/01/19 00:00 [entrez]
AID - gkd179 [pii]
AID - 10.1093/nar/28.3.809 [doi]
PST - ppublish
SO  - Nucleic Acids Res. 2000 Feb 1;28(3):809-17. doi: 10.1093/nar/28.3.809.