PMID- 10637315
OWN - NLM
STAT- MEDLINE
DCOM- 20000407
LR  - 20181113
IS  - 1059-1524 (Print)
IS  - 1059-1524 (Linking)
VI  - 11
IP  - 1
DP  - 2000 Jan
TI  - Association of mouse actin-binding protein 1 (mAbp1/SH3P7), an Src kinase target,
      with dynamic regions of the cortical actin cytoskeleton in response to Rac1
      activation.
PG  - 393-412
AB  - Yeast Abp1p is a cortical actin cytoskeleton protein implicated in cytoskeletal
      regulation, endocytosis, and cAMP-signaling. We have identified a gene encoding a
      mouse homologue of Abp1p, and it is identical to SH3P7, a protein shown recently 
      to be a target of Src tyrosine kinases. Yeast and mouse Abp1p display the same
      domain structure including an N-terminal actin-depolymerizing factor homology
      domain and a C-terminal Src homology 3 domain. Using two independent
      actin-binding domains, mAbp1 binds to actin filaments with a 1:5 saturation
      stoichiometry. In stationary cells, mAbp1 colocalizes with cortical F-actin in
      fibroblast protrusions that represent sites of cellular growth. mAbp1 appears at 
      the actin-rich leading edge of migrating cells. Growth factors cause mAbp1 to
      rapidly accumulate in lamellipodia. This response can be mimicked by expression
      of dominant-positive Rac1. mAbp1 recruitment appears to be dependent on de novo
      actin polymerization and occurs specifically at sites enriched for the Arp2/3
      complex. mAbp1 is a newly identified cytoskeletal protein in mice and may serve
      as a signal-responsive link between the dynamic cortical actin cytoskeleton and
      regions of membrane dynamics.
FAU - Kessels, M M
AU  - Kessels MM
AD  - Department of Molecular and Cell Biology, University of California, Berkeley,
      Berkeley, California 94720-3202, USA.
FAU - Engqvist-Goldstein, A E
AU  - Engqvist-Goldstein AE
FAU - Drubin, D G
AU  - Drubin DG
LA  - eng
GR  - P01 DK032094/DK/NIDDK NIH HHS/United States
GR  - R01 GM050399/GM/NIGMS NIH HHS/United States
GR  - DK-32094/DK/NIDDK NIH HHS/United States
GR  - GM-50399/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Mol Biol Cell
JT  - Molecular biology of the cell
JID - 9201390
RN  - 0 (Actins)
RN  - 0 (Dbnl protein, mouse)
RN  - 0 (HIP-55 protein, human)
RN  - 0 (Microfilament Proteins)
RN  - 0 (Platelet-Derived Growth Factor)
RN  - 0 (Polymers)
RN  - 0 (RNA, Messenger)
RN  - EC 2.7.10.2 (src-Family Kinases)
RN  - EC 3.6.5.2 (rac1 GTP-Binding Protein)
RN  - NI40JAQ945 (Tetradecanoylphorbol Acetate)
SB  - IM
MH  - 3T3 Cells
MH  - Actins/*metabolism
MH  - Amino Acid Sequence
MH  - Animals
MH  - Binding Sites
MH  - COS Cells
MH  - Cell Division
MH  - Cell Line
MH  - Cell Movement
MH  - Cytoskeleton
MH  - Endocytosis
MH  - Humans
MH  - Intracellular Fluid/metabolism
MH  - Mice
MH  - Microfilament Proteins/genetics/*metabolism
MH  - Molecular Sequence Data
MH  - Platelet-Derived Growth Factor/pharmacology
MH  - Polymers
MH  - RNA, Messenger
MH  - Sequence Analysis
MH  - Staining and Labeling
MH  - Tetradecanoylphorbol Acetate/pharmacology
MH  - rac1 GTP-Binding Protein/*metabolism
MH  - *src Homology Domains
MH  - src-Family Kinases/*metabolism
PMC - PMC14781
EDAT- 2000/01/19 00:00
MHDA- 2000/01/19 00:01
CRDT- 2000/01/19 00:00
PHST- 2000/01/19 00:00 [pubmed]
PHST- 2000/01/19 00:01 [medline]
PHST- 2000/01/19 00:00 [entrez]
AID - 10.1091/mbc.11.1.393 [doi]
PST - ppublish
SO  - Mol Biol Cell. 2000 Jan;11(1):393-412. doi: 10.1091/mbc.11.1.393.