PMID- 10637308
OWN - NLM
STAT- MEDLINE
DCOM- 20000407
LR  - 20181113
IS  - 1059-1524 (Print)
IS  - 1059-1524 (Linking)
VI  - 11
IP  - 1
DP  - 2000 Jan
TI  - The N terminus of the transmembrane protein BP180 interacts with the N-terminal
      domain of BP230, thereby mediating keratin cytoskeleton anchorage to the cell
      surface at the site of the hemidesmosome.
PG  - 277-86
AB  - In epidermal cells, the keratin cytoskeleton interacts with the elements in the
      basement membrane via a multimolecular junction called the hemidesmosome. A major
      component of the hemidesmosome plaque is the 230-kDa bullous pemphigoid
      autoantigen (BP230/BPAG1), which connects directly to the keratin-containing
      intermediate filaments of the cytoskeleton via its C terminus. A second bullous
      pemphigoid antigen of 180 kDa (BP180/BPAG2) is a type II transmembrane component 
      of the hemidesmosome. Using yeast two-hybrid technology and recombinant proteins,
      we show that an N-terminal fragment of BP230 can bind directly to an N-terminal
      fragment of BP180. We have also explored the consequences of expression of the
      BP230 N terminus in 804G cells that assemble hemidesmosomes in vitro.
      Unexpectedly, this fragment disrupts the distribution of BP180 in transfected
      cells but has no apparent impact on the organization of endogenous BP230 and
      alpha6beta4 integrin. We propose that the BP230 N terminus competes with
      endogenous BP230 protein for BP180 binding and inhibits incorporation of BP180
      into the cell surface at the site of the hemidesmosome. These data provide new
      insight into those interactions of the molecules of the hemidesmosome that are
      necessary for its function in integrating epithelial and connective tissue types.
FAU - Hopkinson, S B
AU  - Hopkinson SB
AD  - Department of Cell and Molecular Biology, Northwestern University Medical School,
      Chicago, Illinois 60611, USA.
FAU - Jones, J C
AU  - Jones JC
LA  - eng
GR  - R01 GM38470/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Mol Biol Cell
JT  - Molecular biology of the cell
JID - 9201390
RN  - 0 (Autoantigens)
RN  - 0 (Carrier Proteins)
RN  - 0 (Cytoskeletal Proteins)
RN  - 0 (DST protein, human)
RN  - 0 (Dystonin)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (Non-Fibrillar Collagens)
RN  - 0 (collagen type XVII)
RN  - 68238-35-7 (Keratins)
SB  - IM
MH  - Autoantigens/genetics/*metabolism
MH  - Binding Sites
MH  - Carrier Proteins
MH  - Cell Membrane/metabolism
MH  - Cloning, Molecular
MH  - Cytoskeletal Proteins
MH  - Cytoskeleton/metabolism
MH  - Desmosomes/*metabolism
MH  - Dystonin
MH  - Humans
MH  - Keratins/*metabolism
MH  - Nerve Tissue Proteins
MH  - Non-Fibrillar Collagens
MH  - *Pemphigoid, Bullous
MH  - Saccharomyces cerevisiae
MH  - Transfection
PMC - PMC14774
EDAT- 2000/01/19 00:00
MHDA- 2000/01/19 00:01
CRDT- 2000/01/19 00:00
PHST- 2000/01/19 00:00 [pubmed]
PHST- 2000/01/19 00:01 [medline]
PHST- 2000/01/19 00:00 [entrez]
AID - 10.1091/mbc.11.1.277 [doi]
PST - ppublish
SO  - Mol Biol Cell. 2000 Jan;11(1):277-86. doi: 10.1091/mbc.11.1.277.