PMID- 10637234
OWN - NLM
STAT- MEDLINE
DCOM- 20000228
LR  - 20181113
IS  - 0261-4189 (Print)
IS  - 0261-4189 (Linking)
VI  - 19
IP  - 2
DP  - 2000 Jan 17
TI  - A novel RNA binding protein, SBP2, is required for the translation of mammalian
      selenoprotein mRNAs.
PG  - 306-14
AB  - In eukaryotes, the decoding of the UGA codon as selenocysteine (Sec) requires a
      Sec insertion sequence (SECIS) element in the 3' untranslated region of the mRNA.
      We purified a SECIS binding protein, SBP2, and obtained a cDNA clone that encodes
      this activity. SBP2 is a novel protein containing a putative RNA binding domain
      found in ribosomal proteins and a yeast suppressor of translation termination. By
      UV cross-linking and immunoprecipitation, we show that SBP2 specifically binds
      selenoprotein mRNAs both in vitro and in vivo. Using (75)Se-labeled
      Sec-tRNA(Sec), we developed an in vitro system for analyzing Sec incorporation in
      which the translation of a selenoprotein mRNA was both SBP2 and SECIS element
      dependent. Immunodepletion of SBP2 from the lysates abolished Sec insertion,
      which was restored when recombinant SBP2 was added to the reaction. These results
      establish that SBP2 is essential for the co-translational insertion of Sec into
      selenoproteins. We hypothesize that the binding activity of SBP2 may be involved 
      in preventing termination at the UGA/Sec codon.
FAU - Copeland, P R
AU  - Copeland PR
AD  - Department of Cell Biology, Lerner Research Institute, Cleveland Clinic
      Foundation, 9500 Euclid Avenue #NC-10, Cleveland, OH 44195, USA.
FAU - Fletcher, J E
AU  - Fletcher JE
FAU - Carlson, B A
AU  - Carlson BA
FAU - Hatfield, D L
AU  - Hatfield DL
FAU - Driscoll, D M
AU  - Driscoll DM
LA  - eng
SI  - GENBANK/AJ251245
GR  - F32 DK009878/DK/NIDDK NIH HHS/United States
GR  - P01 HL029582/HL/NHLBI NIH HHS/United States
GR  - F32 DK09878-01/DK/NIDDK NIH HHS/United States
GR  - HL29582/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - EMBO J
JT  - The EMBO journal
JID - 8208664
RN  - 0 (Codon)
RN  - 0 (Proteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (RNA-Binding Proteins)
RN  - 0 (Recombinant Proteins)
RN  - 0 (SECISBP2 protein, human)
RN  - 0 (Secisbp2 protein, rat)
RN  - 0 (Selenoproteins)
RN  - 0CH9049VIS (Selenocysteine)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Binding Sites
MH  - Codon/genetics
MH  - Conserved Sequence
MH  - Humans
MH  - Liver Neoplasms, Experimental
MH  - Mammals
MH  - Molecular Sequence Data
MH  - *Protein Biosynthesis
MH  - Proteins/*genetics
MH  - RNA, Messenger/genetics/*metabolism
MH  - RNA-Binding Proteins/chemistry/genetics/*metabolism
MH  - Rats
MH  - Recombinant Proteins/metabolism
MH  - Selenocysteine/genetics/metabolism
MH  - Selenoproteins
MH  - Sequence Alignment
MH  - Sequence Homology, Amino Acid
MH  - Substrate Specificity
MH  - Transfection
MH  - Tumor Cells, Cultured
PMC - PMC305564
EDAT- 2000/01/19 09:00
MHDA- 2000/03/04 09:00
CRDT- 2000/01/19 09:00
PHST- 2000/01/19 09:00 [pubmed]
PHST- 2000/03/04 09:00 [medline]
PHST- 2000/01/19 09:00 [entrez]
AID - 10.1093/emboj/19.2.306 [doi]
PST - ppublish
SO  - EMBO J. 2000 Jan 17;19(2):306-14. doi: 10.1093/emboj/19.2.306.