PMID- 10637231
OWN - NLM
STAT- MEDLINE
DCOM- 20000228
LR  - 20181113
IS  - 0261-4189 (Print)
IS  - 0261-4189 (Linking)
VI  - 19
IP  - 2
DP  - 2000 Jan 17
TI  - A nuclear tyrosine phosphorylation circuit: c-Jun as an activator and substrate
      of c-Abl and JNK.
PG  - 273-81
AB  - The nuclear function of the c-Abl tyrosine kinase is not well understood. In
      order to identify nuclear substrates of Abl, we constructed a constitutively
      active and nuclear form of the protein. We found that active nuclear Abl
      efficiently phosphorylate c-Jun, a transcription factor not previously known to
      be tyrosine phosphorylated. After phosphorylation of c-Jun by Abl on Tyr170, both
      proteins interacted via the SH2 domain of Abl. Surprisingly, elevated levels of
      c-Jun activated nuclear Abl, resulting in activation of the JNK serine/threonine 
      kinase. This phosphorylation circuit generates nuclear tyrosine phosphorylation
      and represents a reversal of previously known signalling models.
FAU - Barila, D
AU  - Barila D
AD  - European Molecular Biology Laboratory, Meyerhofstrasse 1, D-69117 Heidelberg,
      Germany.
FAU - Mangano, R
AU  - Mangano R
FAU - Gonfloni, S
AU  - Gonfloni S
FAU - Kretzschmar, J
AU  - Kretzschmar J
FAU - Moro, M
AU  - Moro M
FAU - Bohmann, D
AU  - Bohmann D
FAU - Superti-Furga, G
AU  - Superti-Furga G
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - EMBO J
JT  - The EMBO journal
JID - 8208664
RN  - 0 (Proto-Oncogene Proteins c-jun)
RN  - 0 (Recombinant Proteins)
RN  - 42HK56048U (Tyrosine)
RN  - EC 2.7.10.2 (Proto-Oncogene Proteins c-abl)
RN  - EC 2.7.11.24 (JNK Mitogen-Activated Protein Kinases)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinases)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Cell Line
MH  - Cell Nucleus/metabolism
MH  - Enzyme Activation
MH  - Humans
MH  - JNK Mitogen-Activated Protein Kinases
MH  - Mice
MH  - Mitogen-Activated Protein Kinases/*metabolism
MH  - Phosphorylation
MH  - Proto-Oncogene Proteins c-abl/*metabolism
MH  - Proto-Oncogene Proteins c-jun/chemistry/*metabolism
MH  - Recombinant Proteins/chemistry/metabolism
MH  - Software
MH  - Substrate Specificity
MH  - Transfection
MH  - Tyrosine
MH  - src Homology Domains
PMC - PMC305561
EDAT- 2000/01/19 09:00
MHDA- 2000/03/04 09:00
CRDT- 2000/01/19 09:00
PHST- 2000/01/19 09:00 [pubmed]
PHST- 2000/03/04 09:00 [medline]
PHST- 2000/01/19 09:00 [entrez]
AID - 10.1093/emboj/19.2.273 [doi]
PST - ppublish
SO  - EMBO J. 2000 Jan 17;19(2):273-81. doi: 10.1093/emboj/19.2.273.