PMID- 10636917
OWN - NLM
STAT- MEDLINE
DCOM- 20000224
LR  - 20190508
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 275
IP  - 3
DP  - 2000 Jan 21
TI  - Identification in vitreous and molecular cloning of opticin, a novel member of
      the family of leucine-rich repeat proteins of the extracellular matrix.
PG  - 2123-9
AB  - A prominent 45-kDa component was identified by protein staining following
      SDS-polyacrylamide gel electrophoresis of a 4 M guanidine hydrochloride extract
      from bovine vitreous collagen fibrils. Peptide sequences obtained from this
      component were used as a basis for the cloning (from human retinal cDNA) and
      sequencing of a novel member of the leucine-rich repeat extracellular matrix
      protein family that we have named opticin. Opticin mRNA was found by reverse
      transcription polymerase chain reaction in ligament and skin as well as in
      retina. An open reading frame containing 332 amino acids was identified, the
      first 19 amino acids representing a signal peptide. The deduced amino acid
      sequence of the mature protein encodes a 35-kDa protein with a calculated
      isoelectric point of 5.4. The central domain of this protein consists of six
      B-type leucine-rich repeats. This domain is flanked by cysteine clusters
      including a C-terminal two-cysteine cluster containing an additional leucine-rich
      repeat. The N-terminal region contains a cluster of potential O-glycosylation
      sites, and analysis of bovine vitreous opticin demonstrated the presence of
      sialylated O-linked oligosaccharides substituting the core protein. Opticin shows
      highest protein sequence identity to epiphycan (42%) and osteoglycin (35%) and
      belongs to Class III of the leucine-rich repeat extracellular matrix protein
      family.
FAU - Reardon, A J
AU  - Reardon AJ
AD  - Wellcome Trust Centre for Cell-Matrix Research, School of Biological Sciences,
      Room 2.14 Stopford Building, University of Manchester, Oxford Road, Manchester
      M13 9PT, United Kingdom.
FAU - Le Goff, M
AU  - Le Goff M
FAU - Briggs, M D
AU  - Briggs MD
FAU - McLeod, D
AU  - McLeod D
FAU - Sheehan, J K
AU  - Sheehan JK
FAU - Thornton, D J
AU  - Thornton DJ
FAU - Bishop, P N
AU  - Bishop PN
LA  - eng
SI  - GENBANK/AJ133790
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (EPYC protein, human)
RN  - 0 (Extracellular Matrix Proteins)
RN  - 0 (Glycoproteins)
RN  - 0 (Intercellular Signaling Peptides and Proteins)
RN  - 0 (OGN protein, human)
RN  - 0 (OPTC protein, human)
RN  - 0 (Peptides)
RN  - 0 (Proteoglycans)
RN  - 0 (Small Leucine-Rich Proteoglycans)
RN  - 9007-34-5 (Collagen)
RN  - GMW67QNF9C (Leucine)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Blotting, Northern
MH  - Cattle
MH  - Collagen/metabolism
MH  - Electrophoresis, Polyacrylamide Gel
MH  - Extracellular Matrix/*chemistry
MH  - Extracellular Matrix Proteins/*chemistry/*genetics/isolation & purification
MH  - Glycoproteins/chemistry
MH  - Humans
MH  - Intercellular Signaling Peptides and Proteins
MH  - Leucine/chemistry
MH  - Male
MH  - Molecular Sequence Data
MH  - Multigene Family
MH  - Peptides/metabolism
MH  - Phylogeny
MH  - Proteoglycans/chemistry
MH  - Retina/chemistry
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Sequence Homology, Amino Acid
MH  - Small Leucine-Rich Proteoglycans
MH  - Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
MH  - Tissue Distribution
MH  - Vitreous Body/*chemistry
EDAT- 2000/01/15 09:00
MHDA- 2000/02/26 09:00
CRDT- 2000/01/15 09:00
PHST- 2000/01/15 09:00 [pubmed]
PHST- 2000/02/26 09:00 [medline]
PHST- 2000/01/15 09:00 [entrez]
AID - 10.1074/jbc.275.3.2123 [doi]
PST - ppublish
SO  - J Biol Chem. 2000 Jan 21;275(3):2123-9. doi: 10.1074/jbc.275.3.2123.