PMID- 10636909
OWN - NLM
STAT- MEDLINE
DCOM- 20000224
LR  - 20210209
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 275
IP  - 3
DP  - 2000 Jan 21
TI  - Palmitoylation targets CD39/endothelial ATP diphosphohydrolase to caveolae.
PG  - 2057-62
AB  - Ectonucleotidases influence purinergic receptor function by the hydrolysis of
      extracellular nucleotides. CD39 is an integral membrane protein that is a
      prototype member of the nucleoside 5'-triphosphate diphosphohydrolase family. The
      native CD39 protein has two intracytoplasmic and two transmembrane domains. There
      is a large extracellular domain that undergoes extensive glycosylation and can be
      post-translationally modified by limited proteolysis. We have identified a
      potential thioester linkage site for S-acylation within the N-terminal region of 
      CD39 and demonstrate that this region undergoes palmitoylation in a constitutive 
      manner. The covalent lipid modification of this region of the protein appears to 
      be important both in plasma membrane association and in targeting CD39 to
      caveolae. These specialized plasmalemmal domains are enriched in G
      protein-coupled receptors and appear to integrate cellular activation events. We 
      suggest that palmitoylation could modulate the function of CD39 in regulating
      cellular signal transduction pathways.
FAU - Koziak, K
AU  - Koziak K
AD  - Department of Medicine, Center for Immunobiology, Beth Israel Deaconess Medical
      Center, Harvard Medical School, Boston, Massachusetts 02215, USA.
FAU - Kaczmarek, E
AU  - Kaczmarek E
FAU - Kittel, A
AU  - Kittel A
FAU - Sevigny, J
AU  - Sevigny J
FAU - Blusztajn, J K
AU  - Blusztajn JK
FAU - Schulte Am Esch, J 2nd
AU  - Schulte Am Esch J 2nd
FAU - Imai, M
AU  - Imai M
FAU - Guckelberger, O
AU  - Guckelberger O
FAU - Goepfert, C
AU  - Goepfert C
FAU - Qawi, I
AU  - Qawi I
FAU - Robson, S C
AU  - Robson SC
LA  - eng
GR  - P01 AG09525/AG/NIA NIH HHS/United States
GR  - R01 HL57307/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (Antigens, CD)
RN  - 2V16EO95H1 (Palmitic Acid)
RN  - EC 3.6.1.- (Adenosine Triphosphatases)
RN  - EC 3.6.1.5 (Apyrase)
RN  - EC 3.6.1.5 (CD39 antigen)
SB  - IM
MH  - *Adenosine Triphosphatases
MH  - Animals
MH  - Antigens, CD/*metabolism
MH  - Apyrase/*metabolism
MH  - Blotting, Western
MH  - COS Cells
MH  - Cell Membrane/metabolism
MH  - Cells, Cultured
MH  - Chromatography, Thin Layer
MH  - Cytoplasm/metabolism
MH  - Endothelium, Vascular/*enzymology/ultrastructure
MH  - Humans
MH  - Microscopy, Electron
MH  - Mutagenesis
MH  - Palmitic Acid/*metabolism
MH  - Signal Transduction
MH  - Transfection
EDAT- 2000/01/15 09:00
MHDA- 2000/02/26 09:00
CRDT- 2000/01/15 09:00
PHST- 2000/01/15 09:00 [pubmed]
PHST- 2000/02/26 09:00 [medline]
PHST- 2000/01/15 09:00 [entrez]
AID - 10.1074/jbc.275.3.2057 [doi]
AID - S0021-9258(18)31142-6 [pii]
PST - ppublish
SO  - J Biol Chem. 2000 Jan 21;275(3):2057-62. doi: 10.1074/jbc.275.3.2057.