PMID- 10636906
OWN - NLM
STAT- MEDLINE
DCOM- 20000224
LR  - 20190508
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 275
IP  - 3
DP  - 2000 Jan 21
TI  - Pantophysin is a phosphoprotein component of adipocyte transport vesicles and
      associates with GLUT4-containing vesicles.
PG  - 2029-36
AB  - Pantophysin, a protein related to the neuroendocrine-specific synaptophysin,
      recently has been identified in non-neuronal tissues. In the present study,
      Northern blots showed that pantophysin mRNA was abundant in adipose tissue and
      increased during adipogenesis of 3T3-L1 cells. Immunoblot analysis of subcellular
      fractions showed pantophysin present exclusively in membrane fractions and
      relatively evenly distributed in the plasma membrane and internal membrane
      fractions. Sucrose gradient ultracentrifugation demonstrated that pantophysin and
      GLUT4 exhibited overlapping distribution profiles. Furthermore, immunopurified
      GLUT4 vesicles contained pantophysin, and both GLUT4 and pantophysin were
      depleted from this vesicle population following treatment with insulin.
      Additionally, a subpopulation of immunopurified pantophysin vesicles contained
      insulin-responsive GLUT4. Consistent with the interaction of synaptophysin with
      vesicle-associated membrane protein 2 in neuroendocrine tissues, pantophysin
      associated with vesicle-associated membrane protein 2 in adipocytes. Furthermore,
      in [(32)P]orthophosphate-labeled cells, pantophysin was phosphorylated in the
      basal state. This phosphorylation was unchanged in response to insulin; however, 
      insulin stimulated the phosphorylation of a 77-kDa protein associated with
      alpha-pantophysin immunoprecipitates. Although the functional role of pantophysin
      in vesicle trafficking is unclear, its presence on GLUT4 vesicles is consistent
      with the emerging role of soluble N-ethylmaleimide-sensitive protein receptor
      (SNARE) factor complex and related proteins in regulated vesicle transport in
      adipocytes. In addition, pantophysin may provide a marker for the analysis of
      other vesicles in adipocytes.
FAU - Brooks, C C
AU  - Brooks CC
AD  - Research Division, Joslin Diabetes Center and Department of Medicine, Harvard
      Medical School, Boston, Massachusetts 02215, USA.
FAU - Scherer, P E
AU  - Scherer PE
FAU - Cleveland, K
AU  - Cleveland K
FAU - Whittemore, J L
AU  - Whittemore JL
FAU - Lodish, H F
AU  - Lodish HF
FAU - Cheatham, B
AU  - Cheatham B
LA  - eng
GR  - DK47618/DK/NIDDK NIH HHS/United States
GR  - DK51668/DK/NIDDK NIH HHS/United States
GR  - HD07938/HD/NICHD NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (Glucose Transporter Type 4)
RN  - 0 (Hypoglycemic Agents)
RN  - 0 (Insulin)
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (Membrane Proteins)
RN  - 0 (Monosaccharide Transport Proteins)
RN  - 0 (Muscle Proteins)
RN  - 0 (R-SNARE Proteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (SLC2A4 protein, human)
RN  - 0 (SNARE Proteins)
RN  - 0 (Slc2a4 protein, mouse)
RN  - 0 (Slc2a4 protein, rat)
RN  - 0 (Synaptophysin)
RN  - 0 (Sypl protein, mouse)
RN  - 0 (Sypl protein, rat)
RN  - 0 (Vesicular Transport Proteins)
RN  - 159447-27-5 (SYPL1 protein, human)
SB  - IM
MH  - 3T3 Cells
MH  - Adipocytes/*metabolism
MH  - Animals
MH  - Biological Transport
MH  - Blotting, Northern
MH  - Cloning, Molecular
MH  - Glucose Transporter Type 4
MH  - Humans
MH  - Hypoglycemic Agents/pharmacology
MH  - Insulin/pharmacology
MH  - Membrane Glycoproteins/*chemistry/*metabolism
MH  - Membrane Proteins/metabolism
MH  - Mice
MH  - Monosaccharide Transport Proteins/*metabolism
MH  - *Muscle Proteins
MH  - Phosphorylation
MH  - Precipitin Tests
MH  - R-SNARE Proteins
MH  - RNA, Messenger/metabolism
MH  - Rats
MH  - SNARE Proteins
MH  - Synaptophysin
MH  - Time Factors
MH  - Tissue Distribution
MH  - Ultracentrifugation
MH  - *Vesicular Transport Proteins
EDAT- 2000/01/15 09:00
MHDA- 2000/02/26 09:00
CRDT- 2000/01/15 09:00
PHST- 2000/01/15 09:00 [pubmed]
PHST- 2000/02/26 09:00 [medline]
PHST- 2000/01/15 09:00 [entrez]
AID - 10.1074/jbc.275.3.2029 [doi]
PST - ppublish
SO  - J Biol Chem. 2000 Jan 21;275(3):2029-36. doi: 10.1074/jbc.275.3.2029.