PMID- 10636902
OWN - NLM
STAT- MEDLINE
DCOM- 20000224
LR  - 20190508
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 275
IP  - 3
DP  - 2000 Jan 21
TI  - A urokinase receptor-associated protein with specific collagen binding
      properties.
PG  - 1993-2002
AB  - The plasminogen activation cascade system, directed by urokinase and the
      urokinase receptor, plays a key role in extracellular proteolysis during tissue
      remodeling. To identify molecular interaction partners of these trigger proteins 
      on the cell, we combined covalent protein cross-linking with mass spectrometry
      based methods for peptide mapping and primary structure analysis of
      electrophoretically isolated protein conjugates. A specific tri-molecular complex
      was observed upon addition of pro-urokinase to human U937 cells. This complex
      included the urokinase receptor, pro-urokinase, and an unknown, high molecular
      weight urokinase receptor-associated protein. The tryptic peptide mixture derived
      from a cross-linked complex of pro-urokinase and the latter protein was analyzed 
      by nanoelectrospray tandem mass spectrometric sequencing. This analysis
      identified the novel protein as the human homologue of a murine membrane-bound
      lectin with hitherto unknown function. The human cDNA was cloned and sequenced.
      The protein, designated uPARAP, is a member of the macrophage mannose receptor
      protein family and contains a putative collagen-binding (fibronectin type II)
      domain in addition to 8 C-type carbohydrate recognition domains. It proved
      capable of binding strongly to a single type of collagen, collagen V. This
      collagen binding reaction at the exact site of plasminogen activation on the cell
      may lead to adhesive functions as well as a contribution to cellular degradation 
      of collagen matrices.
FAU - Behrendt, N
AU  - Behrendt N
AD  - Finsen Laboratory, Rigshospitalet, DK-2100 Copenhagen O, Denmark.
      niels.behrendt@finsenlab.dk
FAU - Jensen, O N
AU  - Jensen ON
FAU - Engelholm, L H
AU  - Engelholm LH
FAU - Mortz, E
AU  - Mortz E
FAU - Mann, M
AU  - Mann M
FAU - Dano, K
AU  - Dano K
LA  - eng
SI  - GENBANK/AF107292
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (Cross-Linking Reagents)
RN  - 0 (MRC2 protein, human)
RN  - 0 (Mannose-Binding Lectins)
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (PLAUR protein, human)
RN  - 0 (Plaur protein, mouse)
RN  - 0 (Receptors, Cell Surface)
RN  - 0 (Receptors, Urokinase Plasminogen Activator)
RN  - 9007-34-5 (Collagen)
RN  - EC 3.4.21.- (Plasminogen Activators)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Cloning, Molecular
MH  - Collagen/*metabolism
MH  - Cross-Linking Reagents/metabolism
MH  - Dose-Response Relationship, Drug
MH  - Glycosylation
MH  - Humans
MH  - *Mannose-Binding Lectins
MH  - Mass Spectrometry
MH  - Membrane Glycoproteins/*chemistry/*metabolism
MH  - Mice
MH  - Molecular Sequence Data
MH  - Muscle, Smooth, Vascular/metabolism
MH  - Plasminogen Activators/metabolism
MH  - Protein Binding
MH  - Protein Structure, Tertiary
MH  - Receptors, Cell Surface/*chemistry/*metabolism
MH  - Receptors, Urokinase Plasminogen Activator
MH  - Sequence Homology, Amino Acid
MH  - U937 Cells
EDAT- 2000/01/15 09:00
MHDA- 2000/02/26 09:00
CRDT- 2000/01/15 09:00
PHST- 2000/01/15 09:00 [pubmed]
PHST- 2000/02/26 09:00 [medline]
PHST- 2000/01/15 09:00 [entrez]
AID - 10.1074/jbc.275.3.1993 [doi]
PST - ppublish
SO  - J Biol Chem. 2000 Jan 21;275(3):1993-2002. doi: 10.1074/jbc.275.3.1993.