PMID- 10636877
OWN - NLM
STAT- MEDLINE
DCOM- 20000224
LR  - 20210209
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 275
IP  - 3
DP  - 2000 Jan 21
TI  - Architecture of high mobility group protein I-C.DNA complex and its perturbation 
      upon phosphorylation by Cdc2 kinase.
PG  - 1793-801
AB  - The high mobility group I-C (HMGI-C) protein is an abundant component of rapidly 
      proliferating undifferentiated cells. High level expression of this protein is
      characteristic for early embryonic tissue and diverse tumors. HMGI-C can function
      as an architectural factor enhancing the activity of transcription factor
      NF-kappaB on the beta-interferon promoter. The protein has three minor groove
      DNA-binding domains (AT-hooks). Here, we describe the complex of HMGI-C with a
      fragment of the beta-interferon promoter. We show that the protein binds to NRDI 
      and PRDII elements of the promoter with its first and second AT-hook,
      respectively. Phosphorylation by Cdc2 kinase leads to a partial derailing of the 
      AT-hooks from the minor groove, affecting mainly the second binding domain. In
      contrast, binding to long AT stretches of DNA involves contacts with all three
      AT-hooks and is marginally sensitive to phosphorylation. Our data stress the
      importance of conformation of the DNA binding site and protein phosphorylation
      for its function.
FAU - Schwanbeck, R
AU  - Schwanbeck R
AD  - III Zoologisches Institut, Entwicklungsbiologie, Universitat Gottingen,
      Humboldtallee 34A, D-37073 Gottingen, Germany.
FAU - Manfioletti, G
AU  - Manfioletti G
FAU - Wisniewski, J R
AU  - Wisniewski JR
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (HMGA2 Protein)
RN  - 0 (High Mobility Group Proteins)
RN  - 77238-31-4 (Interferon-beta)
RN  - 9007-49-2 (DNA)
RN  - EC 2.7.11.22 (CDC2 Protein Kinase)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - CDC2 Protein Kinase/chemistry/*metabolism
MH  - DNA/*metabolism
MH  - DNA Footprinting
MH  - Dose-Response Relationship, Drug
MH  - HMGA2 Protein
MH  - High Mobility Group Proteins/chemistry/genetics/*metabolism
MH  - Interferon-beta/genetics/metabolism
MH  - Methylation
MH  - Mice
MH  - Models, Genetic
MH  - Molecular Sequence Data
MH  - Phosphorylation
MH  - Promoter Regions, Genetic
MH  - Protein Binding
MH  - Protein Conformation
EDAT- 2000/01/15 09:00
MHDA- 2000/02/26 09:00
CRDT- 2000/01/15 09:00
PHST- 2000/01/15 09:00 [pubmed]
PHST- 2000/02/26 09:00 [medline]
PHST- 2000/01/15 09:00 [entrez]
AID - 10.1074/jbc.275.3.1793 [doi]
AID - S0021-9258(18)31110-4 [pii]
PST - ppublish
SO  - J Biol Chem. 2000 Jan 21;275(3):1793-801. doi: 10.1074/jbc.275.3.1793.