PMID- 10635333
OWN - NLM
STAT- MEDLINE
DCOM- 20000131
LR  - 20190915
IS  - 1097-2765 (Print)
IS  - 1097-2765 (Linking)
VI  - 4
IP  - 6
DP  - 1999 Dec
TI  - Phosphorylation of rat serine 105 or mouse threonine 217 in C/EBP beta is
      required for hepatocyte proliferation induced by TGF alpha.
PG  - 1087-92
AB  - We report that TGF alpha induces activation of the p90 ribosomal S kinase (RSK), 
      which results in the phosphorylation of rat C/EBP beta on Ser-105 and of mouse
      C/EBP beta on Thr-217 and concomitantly stimulates proliferation in
      differentiated hepatocytes. Moreover, C/EBP beta-/- mouse hepatocytes respond to 
      TGF alpha when wild-type C/EBP beta is reexpressed, whereas they remain
      refractory to the growth effect of TGF alpha when expressing phosphoacceptor
      mutants rat C/EBP beta Ala-105 or mouse C/EBP beta Ala-217. In contrast, C/EBP
      beta-/- hepatocytes expressing the phosphorylation mimic mutants, rat C/EBP beta 
      Asp-105 or mouse C/EBP beta Glu-217, exhibited marked proliferation in the
      absence of TGF alpha. Thus, a site-specific phosphorylation of the transcription 
      factor C/EBP beta is critical for hepatocyte proliferation induced by TGF alpha
      and other stimuli that activate RSK.
FAU - Buck, M
AU  - Buck M
AD  - Salk Institute for Biological Studies, La Jolla, California 92037, USA.
      mbuck@salk.edu
FAU - Poli, V
AU  - Poli V
FAU - van der Geer, P
AU  - van der Geer P
FAU - Chojkier, M
AU  - Chojkier M
FAU - Hunter, T
AU  - Hunter T
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Mol Cell
JT  - Molecular cell
JID - 9802571
RN  - 0 (CCAAT-Enhancer-Binding Proteins)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Nuclear Proteins)
RN  - 0 (Transforming Growth Factor alpha)
RN  - 2ZD004190S (Threonine)
RN  - 452VLY9402 (Serine)
RN  - EC 2.7.11.1 (Ribosomal Protein S6 Kinases)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - CCAAT-Enhancer-Binding Proteins
MH  - Cell Division/drug effects/physiology
MH  - DNA-Binding Proteins/*physiology
MH  - Enzyme Activation
MH  - Female
MH  - Liver/*pathology/*physiology
MH  - Mice
MH  - Molecular Sequence Data
MH  - Nuclear Proteins/*physiology
MH  - Phosphorylation
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Ribosomal Protein S6 Kinases/*physiology
MH  - Serine
MH  - Signal Transduction/*drug effects
MH  - Threonine
MH  - Transforming Growth Factor alpha/*pharmacology
EDAT- 2000/01/15 00:00
MHDA- 2000/01/15 00:01
CRDT- 2000/01/15 00:00
PHST- 2000/01/15 00:00 [pubmed]
PHST- 2000/01/15 00:01 [medline]
PHST- 2000/01/15 00:00 [entrez]
AID - S1097-2765(00)80237-3 [pii]
AID - 10.1016/s1097-2765(00)80237-3 [doi]
PST - ppublish
SO  - Mol Cell. 1999 Dec;4(6):1087-92. doi: 10.1016/s1097-2765(00)80237-3.