PMID- 10634944
OWN - NLM
STAT- MEDLINE
DCOM- 20000605
LR  - 20190610
IS  - 0006-3002 (Print)
IS  - 0006-3002 (Linking)
VI  - 1483
IP  - 2
DP  - 2000 Jan 17
TI  - The utilization of recombinant prostanoid receptors to determine the affinities 
      and selectivities of prostaglandins and related analogs.
PG  - 285-93
AB  - Stable cell lines that individually express the eight known human prostanoid 
      receptors (EP(1), EP(2), EP(3), EP(4), DP, FP, IP and TP) have been established 
      using human embryonic kidney (HEK) 293(EBNA) cells. These recombinant cell lines 
      have been employed in radioligand binding assays to determine the equilibrium 
      inhibitor constants of known prostanoid receptor ligands at these eight 
      receptors. This has allowed, for the first time, an assessment of the affinity 
      and selectivity of several novel compounds at the individual human prostanoid 
      receptors. This information should facilitate interpretation of pharmacological 
      studies that employ these ligands as tools to study human tissues and cell lines 
      and should, therefore, result in a greater understanding of prostanoid receptor 
      biology.
FAU - Abramovitz, M
AU  - Abramovitz M
AD  - Department of Biochemistry and Molecular Biology, Merck Frosst Centre for 
      Therapeutic Research, Box 1005 Pointe-Claire-, Dorval, Que., Canada.
FAU - Adam, M
AU  - Adam M
FAU - Boie, Y
AU  - Boie Y
FAU - Carriere, M
AU  - Carriere M
FAU - Denis, D
AU  - Denis D
FAU - Godbout, C
AU  - Godbout C
FAU - Lamontagne, S
AU  - Lamontagne S
FAU - Rochette, C
AU  - Rochette C
FAU - Sawyer, N
AU  - Sawyer N
FAU - Tremblay, N M
AU  - Tremblay NM
FAU - Belley, M
AU  - Belley M
FAU - Gallant, M
AU  - Gallant M
FAU - Dufresne, C
AU  - Dufresne C
FAU - Gareau, Y
AU  - Gareau Y
FAU - Ruel, R
AU  - Ruel R
FAU - Juteau, H
AU  - Juteau H
FAU - Labelle, M
AU  - Labelle M
FAU - Ouimet, N
AU  - Ouimet N
FAU - Metters, K M
AU  - Metters KM
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - Netherlands
TA  - Biochim Biophys Acta
JT  - Biochimica et biophysica acta
JID - 0217513
RN  - 0 (Ligands)
RN  - 0 (Prostaglandins)
RN  - 0 (Receptors, Prostaglandin)
RN  - 0 (Recombinant Proteins)
SB  - IM
MH  - Binding, Competitive
MH  - Cell Line
MH  - Cell Membrane/*metabolism
MH  - Humans
MH  - Ligands
MH  - Prostaglandins/*metabolism
MH  - Radioligand Assay
MH  - Receptors, Prostaglandin/agonists/antagonists & inhibitors/*metabolism
MH  - Recombinant Proteins/metabolism
EDAT- 2000/01/15 09:00
MHDA- 2000/06/10 09:00
CRDT- 2000/01/15 09:00
PHST- 2000/01/15 09:00 [pubmed]
PHST- 2000/06/10 09:00 [medline]
PHST- 2000/01/15 09:00 [entrez]
AID - S138819819900164X [pii]
AID - 10.1016/s1388-1981(99)00164-x [doi]
PST - ppublish
SO  - Biochim Biophys Acta. 2000 Jan 17;1483(2):285-93. doi: 
      10.1016/s1388-1981(99)00164-x.