PMID- 10633083
OWN - NLM
STAT- MEDLINE
DCOM- 20000321
LR  - 20181130
IS  - 0021-9533 (Print)
IS  - 0021-9533 (Linking)
VI  - 113 Pt 2
DP  - 2000 Jan
TI  - Isoform specific expression of the neuronal F-actin binding protein, drebrin, in 
      specialized cells of stomach and kidney epithelia.
PG  - 325-36
AB  - To further understand the functional role that the F-actin binding protein,
      drebrin (developmentally regulated brain protein), plays in the regulation of
      F-actin, we characterized its expression in non-neuronal cells. Using
      nanoelectrospray mass spectrometry methods, we initially identified drebrin in
      non-neuronal cultured cells. Using a drebrin-specific monoclonal antibody, we
      were able to detect drebrin protein in several different cell lines derived from 
      fibroblasts, astrocytomas, and simple epithelia, but not in cell lines derived
      from stratified epithelia. Double-label immunofluorescence experiments of
      cultured cell monolayers revealed the localization of drebrin at the apical
      plasma membrane together with a pool of submembranous F-actin. Immunoblot
      analysis of mouse organs revealed that, in addition to its high levels of
      expression in brain, drebrin was present in stomach and to a lesser degree in
      kidney, colon, and urinary bladder. Drebrin protein detected in the non-brain
      organs migrated faster through SDS-PAGE gels, indicating that the lower molecular
      weight embryonic brain isoform (E2) may be the prominent isoform in these organs.
      RT-PCR experiments confirmed the specific expression of the E2 isoform in adult
      stomach, kidney, and cultured cells. In situ immunofluorescence experiments
      revealed a cell-type specific pattern in both stomach and kidney. In stomach,
      drebrin was specifically expressed in the acid-secreting parietal cells of the
      fundic glands, where it accumulated at the extended apical membrane of the
      canaliculi. In kidney, drebrin was expressed in acid-secreting type A
      intercalated cells, where it localized specifically to the apical plasma
      membrane. Drebrin was expressed as well in the distal tubule epithelial cells
      where the protein was concentrated at the luminal surface and present at the
      interdigitations of the basolateral membranes.
FAU - Keon, B H
AU  - Keon BH
AD  - Dept of Cell Biology, Harvard Medical School, Boston, MA 02115, USA.
FAU - Jedrzejewski, P T
AU  - Jedrzejewski PT
FAU - Paul, D L
AU  - Paul DL
FAU - Goodenough, D A
AU  - Goodenough DA
LA  - eng
SI  - GENBANK/AF187147
SI  - GENBANK/AF187148
GR  - GM18974/GM/NIGMS NIH HHS/United States
GR  - GM37751/GM/NIGMS NIH HHS/United States
GR  - T32NS07009/NS/NINDS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - J Cell Sci
JT  - Journal of cell science
JID - 0052457
RN  - 0 (Actins)
RN  - 0 (DNA Primers)
RN  - 0 (DNA, Complementary)
RN  - 0 (Neuropeptides)
RN  - 0 (Protein Isoforms)
RN  - 0 (drebrins)
SB  - IM
MH  - Actins/*metabolism
MH  - Animals
MH  - Base Sequence
MH  - Cell Line
MH  - Cell Membrane/metabolism
MH  - DNA Primers/genetics
MH  - DNA, Complementary/genetics
MH  - Epithelial Cells/metabolism
MH  - Gastric Mucosa/*metabolism
MH  - Humans
MH  - Kidney/*metabolism
MH  - Mass Spectrometry
MH  - Mice
MH  - Molecular Sequence Data
MH  - Neuropeptides/chemistry/genetics/*metabolism
MH  - Protein Isoforms/chemistry/genetics/metabolism
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Tissue Distribution
MH  - Transfection
EDAT- 2000/01/14 09:00
MHDA- 2000/03/25 09:00
CRDT- 2000/01/14 09:00
PHST- 2000/01/14 09:00 [pubmed]
PHST- 2000/03/25 09:00 [medline]
PHST- 2000/01/14 09:00 [entrez]
PST - ppublish
SO  - J Cell Sci. 2000 Jan;113 Pt 2:325-36.