PMID- 10632718
OWN - NLM
STAT- MEDLINE
DCOM- 20000307
LR  - 20190620
IS  - 0014-2956 (Print)
IS  - 0014-2956 (Linking)
VI  - 267
IP  - 2
DP  - 2000 Jan
TI  - Polypeptides differentially expressed in imaginal discs define the peroxiredoxin 
      family of genes in Drosophila.
PG  - 487-97
AB  - 2D gel electrophoresis followed by microsequencing has been used to purify and
      identify a protein (catalogued in the database as SSP5111) from Drosophila wing
      imaginal discs of third instar larvae that showed significant differences in
      their level of expression when compared with other imaginal discs of the same
      age. The microsequence data showed identity with amino acids encoded by the human
      proliferation association gene, pag, which is a thiol-specific antioxidant. By
      virtue of this homology we have cloned and sequenced two cDNAs that appear to
      define the peroxiredoxin family of Drosophila. One of them, Jafrac1, encodes the 
      SSP5111 protein searched, had 194 amino acids and mapped in the region 11E in the
      X chromosome. The other, Jafrac2, encodes a protein of 242 amino acids and mapped
      in the region 62F in the 3 L chromosome. Both new peroxidases contain two
      conserved cysteines and share homology with other peroxidases that extends over
      the entire sequence and ranges between 47% and 76%. An antiserum raised against
      the SSP5111 protein showed significant changes in the amount of protein in
      different stages of Drosophila development, being a major product in early
      embryos. In 2D gels the antibody not only recognizes the SSP5111 polypeptide but 
      also a related one (catalogued in the database as SSP6107) that exhibits
      identical amino-acid sequence over at least 85% of its sequence. The data also
      suggest that the SSP5111 polypeptide could be a maternal-effect product.
FAU - Rodriguez, J
AU  - Rodriguez J
AD  - Centro de Biologia Molecular, Universidad Autonoma de Madrid, Spain.
FAU - Agudo, M
AU  - Agudo M
FAU - Van Damme, J
AU  - Van Damme J
FAU - Vandekerckhove, J
AU  - Vandekerckhove J
FAU - Santaren, J F
AU  - Santaren JF
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Eur J Biochem
JT  - European journal of biochemistry
JID - 0107600
RN  - 0 (Drosophila Proteins)
RN  - 0 (Insect Proteins)
RN  - EC 1.11.1.- (Jafrac1 protein, Drosophila)
RN  - EC 1.11.1.- (Jafrac2 protein, Drosophila)
RN  - EC 1.11.1.- (Peroxidases)
RN  - EC 1.11.1.15 (Peroxiredoxins)
SB  - IM
MH  - Alternative Splicing
MH  - Amino Acid Sequence
MH  - Animals
MH  - Antibody Specificity
MH  - Base Sequence
MH  - Chromosome Mapping
MH  - Cloning, Molecular
MH  - *Drosophila Proteins
MH  - Drosophila melanogaster/embryology/*genetics
MH  - Embryo, Nonmammalian
MH  - Gene Expression Regulation, Developmental
MH  - Insect Proteins/*genetics/immunology/metabolism
MH  - Larva
MH  - Molecular Sequence Data
MH  - Multigene Family
MH  - Peroxidases/*genetics/*metabolism
MH  - Peroxiredoxins
MH  - Protein Biosynthesis
MH  - Sequence Analysis, Protein
MH  - Sequence Homology, Amino Acid
MH  - Species Specificity
MH  - Wings, Animal/embryology
EDAT- 2000/01/13 09:00
MHDA- 2000/03/11 09:00
CRDT- 2000/01/13 09:00
PHST- 2000/01/13 09:00 [pubmed]
PHST- 2000/03/11 09:00 [medline]
PHST- 2000/01/13 09:00 [entrez]
AID - ejb1022 [pii]
AID - 10.1046/j.1432-1327.2000.01022.x [doi]
PST - ppublish
SO  - Eur J Biochem. 2000 Jan;267(2):487-97. doi: 10.1046/j.1432-1327.2000.01022.x.