PMID- 10632593
OWN - NLM
STAT- MEDLINE
DCOM- 20000209
LR  - 20191023
IS  - 1529-2401 (Electronic)
IS  - 0270-6474 (Linking)
VI  - 20
IP  - 2
DP  - 2000 Jan 15
TI  - Identification and characterization of proSAAS, a granin-like neuroendocrine
      peptide precursor that inhibits prohormone processing.
PG  - 639-48
AB  - Five novel peptides were identified in the brains of mice lacking active
      carboxypeptidase E, a neuropeptide-processing enzyme. These peptides are produced
      from a single precursor, termed proSAAS, which is present in human, mouse, and
      rat. ProSAAS mRNA is expressed primarily in brain and other neuroendocrine
      tissues (pituitary, adrenal, pancreas); within brain, the mRNA is broadly
      distributed among neurons. When expressed in AtT-20 cells, proSAAS is secreted
      via the regulated pathway and is also processed at paired-basic cleavage sites
      into smaller peptides. Overexpression of proSAAS in the AtT-20 cells
      substantially reduces the rate of processing of the endogenous prohormone
      proopiomelanocortin. Purified proSAAS inhibits prohormone convertase 1 activity
      with an IC(50) of 590 nM but does not inhibit prohormone convertase 2. Taken
      together, proSAAS may represent an endogenous inhibitor of prohormone convertase 
      1.
FAU - Fricker, L D
AU  - Fricker LD
AD  - Department of Molecular Pharmacology, Albert Einstein College of Medicine, Bronx,
      New York 10461, USA. fricker@aecom.yu.edu
FAU - McKinzie, A A
AU  - McKinzie AA
FAU - Sun, J
AU  - Sun J
FAU - Curran, E
AU  - Curran E
FAU - Qian, Y
AU  - Qian Y
FAU - Yan, L
AU  - Yan L
FAU - Patterson, S D
AU  - Patterson SD
FAU - Courchesne, P L
AU  - Courchesne PL
FAU - Richards, B
AU  - Richards B
FAU - Levin, N
AU  - Levin N
FAU - Mzhavia, N
AU  - Mzhavia N
FAU - Devi, L A
AU  - Devi LA
FAU - Douglass, J
AU  - Douglass J
LA  - eng
GR  - K02DA-00194/DA/NIDA NIH HHS/United States
GR  - R01DA04494/DA/NIDA NIH HHS/United States
GR  - R01NS-26880/NS/NINDS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Neurosci
JT  - The Journal of neuroscience : the official journal of the Society for
      Neuroscience
JID - 8102140
RN  - 0 (Neuropeptides)
RN  - 0 (PCSK1N protein, human)
RN  - 0 (Protein Precursors)
RN  - 0 (RNA, Messenger)
RN  - 0 (Recombinant Proteins)
RN  - 66796-54-1 (Pro-Opiomelanocortin)
RN  - EC 3.4.- (Carboxypeptidases)
RN  - EC 3.4.17.10 (Carboxypeptidase H)
SB  - IM
MH  - Adrenal Glands/metabolism
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Brain/*metabolism
MH  - Carboxypeptidase H
MH  - Carboxypeptidases/deficiency/genetics/*metabolism
MH  - Cell Line
MH  - Humans
MH  - Kinetics
MH  - Mice
MH  - Mice, Mutant Strains
MH  - Molecular Sequence Data
MH  - Neurons/*metabolism
MH  - Neuropeptides/biosynthesis/chemistry/*genetics/*metabolism
MH  - Organ Specificity
MH  - Pancreas/metabolism
MH  - Pituitary Gland/metabolism
MH  - Pro-Opiomelanocortin/genetics/*metabolism
MH  - Protein Precursors/chemistry/*genetics/*metabolism
MH  - *Protein Processing, Post-Translational
MH  - RNA, Messenger/genetics
MH  - Rats
MH  - Recombinant Proteins/metabolism
MH  - Sequence Alignment
MH  - Sequence Homology, Amino Acid
MH  - Transfection
PMC - PMC6772395
EDAT- 2000/01/13 00:00
MHDA- 2000/01/13 00:01
CRDT- 2000/01/13 00:00
PHST- 2000/01/13 00:00 [pubmed]
PHST- 2000/01/13 00:01 [medline]
PHST- 2000/01/13 00:00 [entrez]
PST - ppublish
SO  - J Neurosci. 2000 Jan 15;20(2):639-48.