PMID- 10631978
OWN - NLM
STAT- MEDLINE
DCOM- 20000214
LR  - 20181113
IS  - 0961-8368 (Print)
IS  - 0961-8368 (Linking)
VI  - 8
IP  - 12
DP  - 1999 Dec
TI  - Three-dimensional solution structure of mouse [Cd7]-metallothionein-1 by
      homonuclear and heteronuclear NMR spectroscopy.
PG  - 2630-8
AB  - Sequential 1H-NMR assignments of mouse [Cd7]-metallothionein-1 (MT1) have been
      carried out by standard homonuclear NMR methods and the use of an
      accordion-heteronuclear multiple quantum correlation (HMQC) experiment for
      establishing the metal, 113Cd2+, to cysteine connectivities. The
      three-dimensional structure was then calculated using the distance constraints
      from two-dimensional nuclear Overhauser effect (NOE) spectroscopy spectra and the
      Cys-Cd connectivities as input for a distance geometry-dynamical simulated
      annealing protocol in X-PLOR 3.851. Similar to the mammalian MT2 isoforms, the
      homologous primary structure of MT1 suggested two separate domains, each
      containing one metal cluster. Because there were no interdomain constraints, the 
      structure calculation for the N-terminal beta- and the C-terminal alpha-domain
      were carried out separately. The structures are based on 409 NMR constraints,
      consisting of 381 NOEs and 28 cysteine-metal connectivities. The only elements of
      regular secondary structure found were two short stretches of 3(10) helices along
      with some half-turns in the alpha-domain. Structural comparison with rat liver
      MT2 showed high similarity, with the beta-domain structure in mouse MT1 showing
      evidence of increased flexibility compared to the same domain in MT2. The latter 
      was reflected by the presence of fewer interresidue NOEs, no slowly exchanging
      backbone amide protons, and enhanced cadmium-cadmium exchange rates found in the 
      beta-domain of MT1.
FAU - Zangger, K
AU  - Zangger K
AD  - Department of Biochemistry, Molecular Biology and Biophysics, University of
      Minnesota, Minneapolis 55455, USA.
FAU - Oz, G
AU  - Oz G
FAU - Otvos, J D
AU  - Otvos JD
FAU - Armitage, I M
AU  - Armitage IM
LA  - eng
GR  - DK18778/DK/NIDDK NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Protein Sci
JT  - Protein science : a publication of the Protein Society
JID - 9211750
RN  - 0 (Protein Isoforms)
RN  - 0 (Solutions)
RN  - 00BH33GNGH (Cadmium)
RN  - 9038-94-2 (Metallothionein)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Cadmium/*chemistry
MH  - Magnetic Resonance Spectroscopy
MH  - Metallothionein/*chemistry
MH  - Mice
MH  - Models, Molecular
MH  - Molecular Sequence Data
MH  - Protein Isoforms/chemistry
MH  - Protein Structure, Tertiary
MH  - Rats
MH  - Solutions
PMC - PMC2144227
EDAT- 2000/01/13 09:00
MHDA- 2000/02/19 09:00
CRDT- 2000/01/13 09:00
PHST- 2000/01/13 09:00 [pubmed]
PHST- 2000/02/19 09:00 [medline]
PHST- 2000/01/13 09:00 [entrez]
AID - 10.1110/ps.8.12.2630 [doi]
PST - ppublish
SO  - Protein Sci. 1999 Dec;8(12):2630-8. doi: 10.1110/ps.8.12.2630.