PMID- 10631324
OWN - NLM
STAT- MEDLINE
DCOM- 20000218
LR  - 20190621
IS  - 0014-5793 (Print)
IS  - 0014-5793 (Linking)
VI  - 465
IP  - 2-3
DP  - 2000 Jan 14
TI  - CROP/Luc7A, a novel serine/arginine-rich nuclear protein, isolated from
      cisplatin-resistant cell line.
PG  - 153-6
AB  - A novel putative SR protein, designated cisplatin resistance-associated
      overexpressed protein (CROP), has been cloned from cisplatin-resistant cell lines
      by differential display. The N-half of the deduced amino acid sequence of 432
      amino acids of CROP contains cysteine/histidine motifs and leucine zipper-like
      repeats. The C-half consists mostly of charged and polar amino acids: arginine
      (58 residues or 25%), glutamate (36 residues or 16%), serine (35 residues or
      15%), lysine (30 residues, 13%), and aspartate (20 residues or 9%). The C-half is
      extremely hydrophilic and comprises domains rich in lysine and glutamate
      residues, rich in alternating arginine and glutamate residues, and rich in
      arginine and serine residues. The arginine/serine-rich domain is dominated by a
      series of 8 amino acid imperfect repetitive motif (consensus sequence,
      Ser-Arg-Ser-Arg-Asp/Glu-Arg-Arg-Arg), which has been found in RNA splicing
      factors. The RNase protection assay and Western blotting analysis indicate that
      the expression of CROP is about 2-3-fold higher in mRNA and protein levels in
      cisplatin-resistant ACHN/CDDP cells than in host ACHN cells. CROP is the human
      homologue of yeast Luc7p, which is supposed to be involved in 5'-splice site
      recognition and is essential for vegetative growth.
FAU - Nishii, Y
AU  - Nishii Y
AD  - Laboratory of Biochemistry, Division of Applied Life Sciences, Kyoto University
      Graduate School of Agriculture, Kyoto, Japan.
FAU - Morishima, M
AU  - Morishima M
FAU - Kakehi, Y
AU  - Kakehi Y
FAU - Umehara, K
AU  - Umehara K
FAU - Kioka, N
AU  - Kioka N
FAU - Terano, Y
AU  - Terano Y
FAU - Amachi, T
AU  - Amachi T
FAU - Ueda, K
AU  - Ueda K
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - FEBS Lett
JT  - FEBS letters
JID - 0155157
RN  - 0 (DNA Primers)
RN  - 0 (DNA, Complementary)
RN  - 0 (LUC7A protein, human)
RN  - 0 (Nuclear Proteins)
RN  - 0 (RNA, Messenger)
RN  - Q20Q21Q62J (Cisplatin)
SB  - IM
MH  - Amino Acid Sequence
MH  - Base Sequence
MH  - Cisplatin/*pharmacology
MH  - DNA Primers
MH  - DNA, Complementary
MH  - Humans
MH  - Molecular Sequence Data
MH  - Nuclear Proteins/chemistry/genetics/*isolation & purification
MH  - RNA, Messenger/genetics
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Tumor Cells, Cultured
EDAT- 2000/01/13 09:00
MHDA- 2000/02/26 09:00
CRDT- 2000/01/13 09:00
PHST- 2000/01/13 09:00 [pubmed]
PHST- 2000/02/26 09:00 [medline]
PHST- 2000/01/13 09:00 [entrez]
AID - S0014-5793(99)01744-5 [pii]
AID - 10.1016/s0014-5793(99)01744-5 [doi]
PST - ppublish
SO  - FEBS Lett. 2000 Jan 14;465(2-3):153-6. doi: 10.1016/s0014-5793(99)01744-5.