PMID- 1063125
OWN - NLM
STAT- MEDLINE
DCOM- 19760706
LR  - 20131121
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 251
IP  - 9
DP  - 1976 May 10
TI  - Human deoxythymidine kinase. I. Purification and general properties of the
      cytoplasmic and mitochondrial isozymes derived from blast cells of acute
      myelocytic leukemia.
PG  - 2600-4
AB  - Two forms of deoxythymidine kinase from blast cells of acute myelocytic leukemia 
      were identified by electrophoresis. One was associated mainly with the cytoplasm 
      and the other with mitochondria. Both isozymes were separated and purified by
      differential affinity column chromatography which resulted in 2416- and 1634-fold
      purification of the cytoplasmic and mitochondrial enzymes, respectively. Affinity
      gel was prepared by linkage through position 3' of deoxythymidine. Each enzyme
      had the same electrophoretic mobility in the purified state as it did in the
      enzyme derived from the corresponding subcellular fraction of the homogenate.
      Thymidine phosphorylase was not retarded by the affinity column. The purified
      cytoplasmic and mitochondrial deoxythymidine kinase had different molecular
      weights, sensitivities to inhibition by ammonium sulfate, activation energies for
      the reaction and divalent cation requirements. Adenosine, guanosine, and cytosine
      3':5'-monophosphates, putrescine, spermine, and spermidine were neither
      activators nor inhibitors of either deoxythymidine kinase.
FAU - Lee, L S
AU  - Lee LS
FAU - Cheng, Y C
AU  - Cheng YC
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (Cations, Divalent)
RN  - 0 (Isoenzymes)
RN  - EC 2.7.1.21 (Thymidine Kinase)
RN  - SU46BAM238 (Ammonium Sulfate)
SB  - IM
MH  - Ammonium Sulfate/pharmacology
MH  - Cations, Divalent
MH  - Chromatography, Affinity
MH  - Cytoplasm/enzymology
MH  - Humans
MH  - Isoenzymes/*blood/isolation & purification/metabolism
MH  - Kinetics
MH  - Leukemia, Myeloid, Acute/*enzymology
MH  - Leukocytes/*enzymology
MH  - Mitochondria/*enzymology
MH  - Molecular Weight
MH  - Osmolar Concentration
MH  - Temperature
MH  - Thymidine Kinase/*blood/isolation & purification/metabolism
EDAT- 1976/05/10 00:00
MHDA- 1976/05/10 00:01
CRDT- 1976/05/10 00:00
PHST- 1976/05/10 00:00 [pubmed]
PHST- 1976/05/10 00:01 [medline]
PHST- 1976/05/10 00:00 [entrez]
PST - ppublish
SO  - J Biol Chem. 1976 May 10;251(9):2600-4.