PMID- 10631117
OWN - NLM
STAT- MEDLINE
DCOM- 20000223
LR  - 20081121
IS  - 0006-291X (Print)
IS  - 0006-291X (Linking)
VI  - 267
IP  - 2
DP  - 2000 Jan 19
TI  - Characterization of the promoter of the human ribonucleotide reductase R2 gene.
PG  - 651-7
AB  - We describe here cloning and characterization of the promoter region of the human
      ribonucleotide reductase R2 gene. Primer extension and sequence data indicated
      that two different transcripts were produced via using two different promoter
      regions. Promoter activity of the 5' flanking region of the first transcript was 
      approximately 100-fold higher than controls, and that of the second transcript
      was approximately 30-fold higher than controls. Particularly, the proximal region
      of the first transcript, -125 to +1 bp, was responsible for approximately a
      50-fold increase in promoter activity, compared to controls. This region had
      three CCAAT sequences, each of which contributed similarly to promoter activity. 
      When all three CCAAT sequences were mutated, promoter activity declined 80%. In
      addition, the promoter region -125 to +1 bp was responsible for
      cell-cycle-specific expression. These data provided essential information
      concerning regulatory mechanisms of cell-cycle-specific expression of human
      ribonucleotide reductase R2.
CI  - Copyright 2000 Academic Press.
FAU - Park, J B
AU  - Park JB
AD  - Phytonutrients Laboratory, BHNRC/ARS/USDA, Building 307, Room 313, Beltsville,
      Maryland 20705, USA.
FAU - Levine, M
AU  - Levine M
LA  - eng
SI  - GENBANK/AF149206
PT  - Journal Article
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (DNA Primers)
RN  - 9007-49-2 (DNA)
RN  - EC 1.17.4.- (Ribonucleotide Reductases)
RN  - EC 1.17.4.- (ribonucleotide reductase R2 subunit)
SB  - IM
MH  - Base Sequence
MH  - Binding Sites/genetics
MH  - Cell Cycle
MH  - Cloning, Molecular
MH  - DNA/genetics
MH  - DNA Primers/genetics
MH  - Gene Expression
MH  - Humans
MH  - Molecular Sequence Data
MH  - *Promoter Regions, Genetic
MH  - Ribonucleotide Reductases/*genetics
EDAT- 2000/01/13 09:00
MHDA- 2000/02/26 09:00
CRDT- 2000/01/13 09:00
PHST- 2000/01/13 09:00 [pubmed]
PHST- 2000/02/26 09:00 [medline]
PHST- 2000/01/13 09:00 [entrez]
AID - 10.1006/bbrc.1999.1980 [doi]
AID - S0006-291X(99)91980-0 [pii]
PST - ppublish
SO  - Biochem Biophys Res Commun. 2000 Jan 19;267(2):651-7. doi:
      10.1006/bbrc.1999.1980.