PMID- 10631108
OWN - NLM
STAT- MEDLINE
DCOM- 20000223
LR  - 20061115
IS  - 0006-291X (Print)
IS  - 0006-291X (Linking)
VI  - 267
IP  - 2
DP  - 2000 Jan 19
TI  - Establishment of the anti-Klotho monoclonal antibodies and detection of Klotho
      protein in kidneys.
PG  - 597-602
AB  - A novel gene, klotho (kl), which is involved in the development of a syndrome
      resembling human aging in mice, was recently identified. The kl gene encodes a
      single-pass membrane protein whose extracellular domain carries homology to
      beta-glucosidases. There also exists a splice variant of kl mRNA which encodes a 
      putative secreted protein in both human and mouse. In this study, to characterize
      the physiological roles of Klotho protein, we established three monoclonal
      antibodies (mAbs) against the recombinant human Klotho protein. The mAbs are
      named KM2076 (rat IgG(2)a), KM2119 (rat IgG(2)b), and KM2365 (mouse IgG(1)). In
      Western blots, KM2076 and KM2119 specifically recognized a 130 kDa Klotho protein
      in the mouse and human kidney membrane fractions. To detect the human Klotho
      protein, the sandwich-type ELISA system with KM2076 and KM2365 was established.
      Using the ELISA system, we detected the human Klotho protein as low as 20 ng/ml
      in the supernatant of Chinese hamster ovary cells (CHO cells), introduced the
      human klotho gene. KM2076 and KM2119 specifically gave a positive staining by
      immunohistochemical staining in paraffin or frozen sections of the kidneys from
      wild-type mice but not in those from kl mice. Strong staining was observed
      especially in cortical renal tubules of the mouse kidney, where expression of
      klotho transcripts overlaps. KM2076 also showed a similar reaction pattern in the
      paraffin sections of rat and human kidneys. The mAbs established in this paper
      will serve as useful analytical, pathological, and diagnostic tools to disclose
      the role of Klotho protein in the suppression of a syndrome resembling human
      aging.
CI  - Copyright 2000 Academic Press.
FAU - Kato, Y
AU  - Kato Y
AD  - Tokyo Research Laboratories, Kyowa Hakko Kogyo Co. Ltd., 3-6-6, Asahi-machi,
      Machida-shi, Tokyo, 194-8533, Japan.
FAU - Arakawa, E
AU  - Arakawa E
FAU - Kinoshita, S
AU  - Kinoshita S
FAU - Shirai, A
AU  - Shirai A
FAU - Furuya, A
AU  - Furuya A
FAU - Yamano, K
AU  - Yamano K
FAU - Nakamura, K
AU  - Nakamura K
FAU - Iida, A
AU  - Iida A
FAU - Anazawa, H
AU  - Anazawa H
FAU - Koh, N
AU  - Koh N
FAU - Iwano, A
AU  - Iwano A
FAU - Imura, A
AU  - Imura A
FAU - Fujimori, T
AU  - Fujimori T
FAU - Kuro-o, M
AU  - Kuro-o M
FAU - Hanai, N
AU  - Hanai N
FAU - Takeshige, K
AU  - Takeshige K
FAU - Nabeshima, Y
AU  - Nabeshima Y
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Membrane Proteins)
RN  - 0 (Peptide Fragments)
RN  - 0 (RNA, Messenger)
RN  - 0 (Recombinant Proteins)
RN  - EC 3.2.1.31 (Glucuronidase)
RN  - EC 3.2.1.31 (klotho protein)
SB  - IM
MH  - Aging/genetics/metabolism
MH  - Alternative Splicing
MH  - Amino Acid Sequence
MH  - Animals
MH  - *Antibodies, Monoclonal
MH  - CHO Cells
MH  - Cricetinae
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Glucuronidase
MH  - Humans
MH  - Immunohistochemistry
MH  - In Situ Hybridization
MH  - Kidney/*metabolism
MH  - Membrane Proteins/genetics/*immunology/*metabolism
MH  - Mice
MH  - Molecular Sequence Data
MH  - Peptide Fragments/genetics/immunology
MH  - RNA, Messenger/genetics/metabolism
MH  - Rats
MH  - Recombinant Proteins/genetics/immunology/metabolism
EDAT- 2000/01/13 09:00
MHDA- 2000/02/26 09:00
CRDT- 2000/01/13 09:00
PHST- 2000/01/13 09:00 [pubmed]
PHST- 2000/02/26 09:00 [medline]
PHST- 2000/01/13 09:00 [entrez]
AID - 10.1006/bbrc.1999.2009 [doi]
AID - S0006291X99920090 [pii]
PST - ppublish
SO  - Biochem Biophys Res Commun. 2000 Jan 19;267(2):597-602. doi:
      10.1006/bbrc.1999.2009.