PMID- 10630373
OWN - NLM
STAT- MEDLINE
DCOM- 20000210
LR  - 20061115
IS  - 1226-3613 (Print)
IS  - 1226-3613 (Linking)
VI  - 31
IP  - 4
DP  - 1999 Dec 31
TI  - Molecular cloning of clathrin assembly protein gene (rCALM) and its differential 
      expression to AP180 in rat brain.
PG  - 191-6
AB  - Binding of clathrin assembly protein to clathrin triskelia induces their assembly
      into clathrin-coated vesicle (CCV) in neurons. The clathrin assembly protein gene
      (rCALM) was cloned from rat brain cDNA library. rCALM deduced 69 kD molecule has 
      overall 73% amino acid homology compared with that of AP180 protein. The
      N-terminal domain, where amino acid sequences are very similar with AP180,
      harbours binding sites for clathrin and inositides, as well as possible
      phosphorylation sites, but the proline rich C-terminal domain is different from
      that of AP180. The mRNA expression of rCALM and AP180 by in situ hybridization
      histochemistry revealed that the rCALM mRNA was more intensely expressed than
      that of AP180, and the distribution patterns were different from each other.
      These results suggest that the rCALM mediates the assembly of clathrin in neural 
      and supporting cells of brain, and regulates the clathrin coated-vesicle
      formation through phosphorylation and inositide metabolism.
FAU - Kim, H L
AU  - Kim HL
AD  - Department of Biochemistry, College of Medicine, Ewha Womans University, Seoul,
      Korea. hyung@mm.ewha.ac.kr
FAU - Lee, S C
AU  - Lee SC
LA  - eng
SI  - GENBANK/AF041373
SI  - GENBANK/AF041374
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Exp Mol Med
JT  - Experimental & molecular medicine
JID - 9607880
RN  - 0 (Adaptor Proteins, Vesicular Transport)
RN  - 0 (Monomeric Clathrin Assembly Proteins)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (Phosphoproteins)
RN  - 0 (clathrin assembly protein AP180)
SB  - IM
MH  - Adaptor Proteins, Vesicular Transport
MH  - Age Factors
MH  - Alternative Splicing
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Brain/*physiology
MH  - Cloning, Molecular
MH  - Gene Expression Regulation, Developmental
MH  - Molecular Sequence Data
MH  - *Monomeric Clathrin Assembly Proteins
MH  - Nerve Tissue Proteins/*genetics/metabolism
MH  - Phosphoproteins/*genetics/metabolism
MH  - Rats
MH  - Sequence Homology, Amino Acid
EDAT- 2000/01/12 00:00
MHDA- 2000/01/12 00:01
CRDT- 2000/01/12 00:00
PHST- 2000/01/12 00:00 [pubmed]
PHST- 2000/01/12 00:01 [medline]
PHST- 2000/01/12 00:00 [entrez]
AID - 10.1038/emm.1999.31 [doi]
PST - ppublish
SO  - Exp Mol Med. 1999 Dec 31;31(4):191-6. doi: 10.1038/emm.1999.31.