PMID- 10630292
OWN - NLM
STAT- MEDLINE
DCOM- 20000127
LR  - 20190831
IS  - 0093-7711 (Print)
IS  - 0093-7711 (Linking)
VI  - 50
IP  - 5-6
DP  - 1999 Dec
TI  - The gp49A gene has extensive sequence conservation with the gp49B gene and
      provides gp49A protein, a unique member of a large family of activating and
      inhibitory receptors of the immunoglobulin superfamily.
PG  - 286-94
AB  - Members of the gp49-related family of mouse and human immunoglobulin (Ig)
      superfamily receptors have significant amino acid sequence homology in their
      C2-type, Ig-like domains and include the killer cell Ig-like receptors (KIRs) for
      major histocompatibility complex class I molecules. We now report the cloning,
      complete sequence, and organization of the mouse gp49A gene that encodes the only
      member of this newly-appreciated family without either of two mutually exclusive 
      functional motifs, namely, immunoreceptor tyrosine-based inhibitory motifs
      (ITIMs) or a charged transmembrane amino acid for heterodimerization with
      activation molecules. The gp49A and gp49B genes are 94% identical over 5.6
      kilobases, the 5' flanking regions are 94% identical over 1900 nucleotides, and
      the 3' flanking regions are 97% identical for 121 nucleotides and then diverge
      completely; the gp49B gene encodes gp49B1 bearing two ITIMs. As measured by flow 
      cytometry with specific antibody, gp49A is expressed on immature
      bone-marrow-derived mast cells, mature serosal mast cells, and several mouse mast
      cell lines. The substantial sequence identity of the introns of the gp49A and
      gp49B genes is comparable to that of the exons, establishing the gene pair as the
      most homologous of the gp49-related family and suggesting that the gp49A and
      gp49B genes arose by duplication with relatively little subsequent mutation. The 
      findings also represent the first demonstration that gp49A is expressed on mast
      cells in tandem with inhibitory gp49B1, and establish that the gp49A gene is not 
      a pseudogene, but rather encodes a protein product with characteristics different
      from the other family members.
FAU - McCormick, M J
AU  - McCormick MJ
AD  - Department of Medicine, Harvard Medical School, Brigham and Women's Hospital,
      Boston, MA 02115, USA.
FAU - Castells, M C
AU  - Castells MC
FAU - Austen, K F
AU  - Austen KF
FAU - Katz, H R
AU  - Katz HR
LA  - eng
SI  - GENBANK/AF141314
GR  - AI-07306/AI/NIAID NIH HHS/United States
GR  - AI-22531/AI/NIAID NIH HHS/United States
GR  - AI-31599/AI/NIAID NIH HHS/United States
GR  - etc.
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Immunogenetics
JT  - Immunogenetics
JID - 0420404
RN  - 0 (Antigens, Surface)
RN  - 0 (DNA, Complementary)
RN  - 0 (Gp49a protein, mouse)
RN  - 0 (Immunoglobulins)
RN  - 0 (Lilrb4 protein, mouse)
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (Receptors, Immunologic)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Antigens, Surface/*biosynthesis/immunology/metabolism
MH  - Base Sequence
MH  - Bone Marrow/metabolism
MH  - Cell Line
MH  - Cells, Cultured
MH  - Conserved Sequence
MH  - DNA, Complementary/metabolism
MH  - Exons
MH  - Flow Cytometry
MH  - Humans
MH  - Immunoglobulins/genetics
MH  - Introns
MH  - Male
MH  - Mast Cells/metabolism
MH  - Membrane Glycoproteins/*genetics/*metabolism
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Models, Genetic
MH  - Receptors, Immunologic/*genetics
MH  - Transfection
EDAT- 2000/01/12 00:00
MHDA- 2000/01/12 00:01
CRDT- 2000/01/12 00:00
PHST- 2000/01/12 00:00 [pubmed]
PHST- 2000/01/12 00:01 [medline]
PHST- 2000/01/12 00:00 [entrez]
AID - 10.1007/s002510050604 [doi]
PST - ppublish
SO  - Immunogenetics. 1999 Dec;50(5-6):286-94. doi: 10.1007/s002510050604.