PMID- 10629066
OWN - NLM
STAT- MEDLINE
DCOM- 20000214
LR  - 20190508
IS  - 0270-7306 (Print)
IS  - 0270-7306 (Linking)
VI  - 20
IP  - 3
DP  - 2000 Feb
TI  - Targeted disruption of mouse fibroblast activation protein.
PG  - 1089-94
AB  - Human fibroblast activation protein (FAP), a member of the serine prolyl
      oligopeptidase family, is a type II cell surface glycoprotein selectively
      expressed by fibroblastic cells in areas of active tissue remodeling, such as the
      embryonic mesenchyme, areas of wound healing, the gravid uterus, and the reactive
      stroma of epithelial cancers. Homologues of FAP have been identified in the mouse
      and Xenopus laevis. FAP is a dual-specificity enzyme that acts as a dipeptidyl
      peptidase and collagenase in vitro. To explore the role of FAP in vivo, Fap(-/-) 
      mice were generated by homologous recombination. RNase protection analysis and
      reverse transcription-PCR confirmed the absence of full-length Fap transcripts in
      mouse embryonic tissues. No FAP protein was detected in Fap(-/-) animals by
      immunohistochemistry, and no FAP-specific dipeptidyl peptidase activity was
      found. We report that Fap(-/-) mice are fertile, show no overt developmental
      defects, and have no general change in cancer susceptibility.
FAU - Niedermeyer, J
AU  - Niedermeyer J
AD  - Department of Oncology Research, Boehringer Ingelheim Pharma KG, 88397 Biberach, 
      Germany.
FAU - Kriz, M
AU  - Kriz M
FAU - Hilberg, F
AU  - Hilberg F
FAU - Garin-Chesa, P
AU  - Garin-Chesa P
FAU - Bamberger, U
AU  - Bamberger U
FAU - Lenter, M C
AU  - Lenter MC
FAU - Park, J
AU  - Park J
FAU - Viertel, B
AU  - Viertel B
FAU - Puschner, H
AU  - Puschner H
FAU - Mauz, M
AU  - Mauz M
FAU - Rettig, W J
AU  - Rettig WJ
FAU - Schnapp, A
AU  - Schnapp A
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Mol Cell Biol
JT  - Molecular and cellular biology
JID - 8109087
RN  - 0 (Antigens, Neoplasm)
RN  - 0 (Biomarkers, Tumor)
RN  - 0 (Growth Substances)
RN  - 0 (Membrane Proteins)
RN  - EC 3.4.21.- (Serine Endopeptidases)
RN  - EC 3.4.21.- (fibroblast activation protein alpha)
RN  - EC 3.4.24.- (Gelatinases)
SB  - IM
MH  - Aging
MH  - Animals
MH  - *Antigens, Neoplasm
MH  - *Biomarkers, Tumor
MH  - Crosses, Genetic
MH  - Embryo, Mammalian
MH  - Embryo, Nonmammalian
MH  - Embryonic and Fetal Development
MH  - Female
MH  - Fertility
MH  - Fibroblasts/metabolism
MH  - Gelatinases
MH  - Growth Substances/deficiency/*genetics/*metabolism
MH  - Humans
MH  - Male
MH  - Membrane Proteins
MH  - Mesoderm/cytology/physiology
MH  - Mice
MH  - Mice, Inbred Strains
MH  - Mice, Knockout
MH  - Recombination, Genetic
MH  - Restriction Mapping
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Serine Endopeptidases/deficiency/*genetics/*metabolism
MH  - Stem Cells
MH  - *Transcription, Genetic
MH  - Xenopus laevis
PMC - PMC85226
EDAT- 2000/01/11 09:00
MHDA- 2000/02/19 09:00
CRDT- 2000/01/11 09:00
PHST- 2000/01/11 09:00 [pubmed]
PHST- 2000/02/19 09:00 [medline]
PHST- 2000/01/11 09:00 [entrez]
AID - 10.1128/mcb.20.3.1089-1094.2000 [doi]
PST - ppublish
SO  - Mol Cell Biol. 2000 Feb;20(3):1089-94. doi: 10.1128/mcb.20.3.1089-1094.2000.