PMID- 10629062
OWN - NLM
STAT- MEDLINE
DCOM- 20000214
LR  - 20190508
IS  - 0270-7306 (Print)
IS  - 0270-7306 (Linking)
VI  - 20
IP  - 3
DP  - 2000 Feb
TI  - Peri-implantation lethality in mice lacking the Sm motif-containing protein Lsm4.
PG  - 1055-62
AB  - Small nuclear ribonucleoproteins (snRNPs) are particles present only in
      eukaryotic cells. They are involved in a large variety of RNA maturation
      processes, most notably in pre-mRNA splicing. Several of the proteins typically
      found in snRNPs contain a sequence signature, the Sm domain, conserved from yeast
      to mammals. By using a promoter trap strategy to target actively transcribed loci
      in murine embryonic stem cells, a new murine gene encoding an Sm motif-containing
      protein was identified. Database searches revealed that it is the mouse
      orthologue of Lsm4p, a protein found in yeast and human cells and putatively
      associated with U6 snRNA. Introduction of the geo reporter gene cassette under
      the control of the murine Lsm4 (mLsm4) endogenous promoter showed that the gene
      was ubiquitously transcribed in embryonic and adult tissues. The insertion of the
      geo cassette disrupted the mLsm4 allele, and homozygosity for the mutation led to
      a recessive embryonic lethal phenotype. mLsm4-null zygotes survived to the
      blastocyst stages, implanted into the uterus, but died shortly thereafter. The
      early death of mLsm4p-null mice suggests that the role of mLsm4p in splicing is
      essential and cannot be compensated by other Lsm proteins.
FAU - Hirsch, E
AU  - Hirsch E
AD  - Max Planck Institut fur Biochemie, 82152 Martinsried, Germany.
FAU - Oohashi, T
AU  - Oohashi T
FAU - Ahmad, M
AU  - Ahmad M
FAU - Stamm, S
AU  - Stamm S
FAU - Fassler, R
AU  - Fassler R
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Mol Cell Biol
JT  - Molecular and cellular biology
JID - 8109087
RN  - 0 (LSM4 protein, human)
RN  - 0 (Lsm4 protein, mouse)
RN  - 0 (RNA, Small Nuclear)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 0 (Ribonucleoproteins, Small Nuclear)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Cell Line
MH  - Cloning, Molecular
MH  - *Embryo Implantation
MH  - Embryonic and Fetal Development
MH  - Female
MH  - Fetal Death
MH  - Gene Expression Regulation, Developmental
MH  - Genes, Reporter
MH  - Humans
MH  - Mice
MH  - Molecular Sequence Data
MH  - *Promoter Regions, Genetic
MH  - RNA, Small Nuclear/*metabolism
MH  - Recombinant Fusion Proteins/biosynthesis
MH  - Restriction Mapping
MH  - Ribonucleoproteins, Small Nuclear/deficiency/*genetics/*metabolism
MH  - Saccharomyces cerevisiae/genetics
MH  - Schizosaccharomyces/genetics
MH  - Sequence Alignment
MH  - *Sequence Deletion
MH  - Sequence Homology, Amino Acid
MH  - Stem Cells/physiology
MH  - Transcription, Genetic
MH  - Transfection
PMC - PMC85222
EDAT- 2000/01/11 09:00
MHDA- 2000/02/19 09:00
CRDT- 2000/01/11 09:00
PHST- 2000/01/11 09:00 [pubmed]
PHST- 2000/02/19 09:00 [medline]
PHST- 2000/01/11 09:00 [entrez]
AID - 10.1128/mcb.20.3.1055-1062.2000 [doi]
PST - ppublish
SO  - Mol Cell Biol. 2000 Feb;20(3):1055-62. doi: 10.1128/mcb.20.3.1055-1062.2000.