PMID- 10629059
OWN - NLM
STAT- MEDLINE
DCOM- 20000214
LR  - 20220408
IS  - 0270-7306 (Print)
IS  - 0270-7306 (Linking)
VI  - 20
IP  - 3
DP  - 2000 Feb
TI  - PR48, a novel regulatory subunit of protein phosphatase 2A, interacts with Cdc6
      and modulates DNA replication in human cells.
PG  - 1021-9
AB  - Initiation of DNA replication in eukaryotes is dependent on the activity of
      protein phosphatase 2A (PP2A), but specific phosphoprotein substrates pertinent
      to this requirement have not been identified. A novel regulatory subunit of PP2A,
      termed PR48, was identified by a yeast two-hybrid screen of a human placental
      cDNA library, using human Cdc6, an essential component of prereplicative
      complexes, as bait. PR48 binds specifically to an amino-terminal segment of Cdc6 
      and forms functional holoenzyme complexes with A and C subunits of PP2A. PR48
      localizes to the nucleus of mammalian cells, and its forced overexpression
      perturbs cell cycle progression, causing a G(1) arrest. These results suggest
      that dephosphorylation of Cdc6 by PP2A, mediated by a specific interaction with
      PR48, is a regulatory event controlling initiation of DNA replication in
      mammalian cells.
FAU - Yan, Z
AU  - Yan Z
AD  - Department of Internal Medicine, University of Texas Southwestern Medical Center,
      Dallas, Texas 75390, USA.
FAU - Fedorov, S A
AU  - Fedorov SA
FAU - Mumby, M C
AU  - Mumby MC
FAU - Williams, R S
AU  - Williams RS
LA  - eng
GR  - HLK31107/HL/NHLBI NIH HHS/United States
GR  - T32 HL007360/HL/NHLBI NIH HHS/United States
GR  - HL06296/HL/NHLBI NIH HHS/United States
GR  - HL07360/HL/NHLBI NIH HHS/United States
GR  - P01 HL006296/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Mol Cell Biol
JT  - Molecular and cellular biology
JID - 8109087
RN  - 0 (CDC6 protein, S cerevisiae)
RN  - 0 (Cell Cycle Proteins)
RN  - 0 (Macromolecular Substances)
RN  - 0 (PPP2R3B protein, human)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 0 (Saccharomyces cerevisiae Proteins)
RN  - EC 3.1.3.16 (Phosphoprotein Phosphatases)
RN  - EC 3.1.3.16 (Protein Phosphatase 2)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Cell Cycle Proteins/*metabolism
MH  - Cell Line
MH  - Cloning, Molecular
MH  - *DNA Replication
MH  - Female
MH  - Gene Library
MH  - HeLa Cells
MH  - Humans
MH  - Macromolecular Substances
MH  - Mice
MH  - Molecular Sequence Data
MH  - Phosphoprotein Phosphatases/chemistry/genetics/*metabolism
MH  - Phylogeny
MH  - Placenta/enzymology
MH  - Pregnancy
MH  - Protein Phosphatase 2
MH  - Protein Structure, Quaternary
MH  - Rabbits
MH  - Recombinant Fusion Proteins/metabolism
MH  - Saccharomyces cerevisiae/genetics/growth & development
MH  - *Saccharomyces cerevisiae Proteins
MH  - Sequence Alignment
MH  - Sequence Homology, Amino Acid
MH  - Spodoptera
MH  - Transfection
PMC - PMC85219
EDAT- 2000/01/11 09:00
MHDA- 2000/02/19 09:00
CRDT- 2000/01/11 09:00
PHST- 2000/01/11 09:00 [pubmed]
PHST- 2000/02/19 09:00 [medline]
PHST- 2000/01/11 09:00 [entrez]
AID - 10.1128/MCB.20.3.1021-1029.2000 [doi]
PST - ppublish
SO  - Mol Cell Biol. 2000 Feb;20(3):1021-9. doi: 10.1128/MCB.20.3.1021-1029.2000.