PMID- 10629049
OWN - NLM
STAT- MEDLINE
DCOM- 20000214
LR  - 20210526
IS  - 0270-7306 (Print)
IS  - 0270-7306 (Linking)
VI  - 20
IP  - 3
DP  - 2000 Feb
TI  - Mutations in host cell factor 1 separate its role in cell proliferation from
      recruitment of VP16 and LZIP.
PG  - 919-28
AB  - Host cell factor 1 (HCF-1) is a nuclear protein required for progression through 
      G(1) phase of the cell cycle and, via its association with VP16, transcriptional 
      activation of the herpes simplex virus immediate-early genes. Both functions
      require a six-bladed beta-propeller domain encoded by residues 1 to 380 of HCF-1 
      as well as an additional amino-terminal region. The beta-propeller domain is well
      conserved in HCF homologues, consistent with a critical cellular function. To
      date, the only known cellular target of the beta-propeller is a bZIP
      transcription factor known as LZIP or Luman. Whether the interaction between
      HCF-1 and LZIP is required for cell proliferation remains to be determined. In
      this study, we used directed mutations to show that all six blades of the HCF-1
      beta-propeller contribute to VP16-induced complex assembly, association with
      LZIP, and cell cycle progression. Although LZIP and VP16 share a common
      tetrapeptide HCF-binding motif, our results reveal profound differences in their 
      interaction with HCF-1. Importantly, with several of the mutants we observe a
      poor correlation between the ability to associate with LZIP and promote cell
      proliferation in the context of the full HCF-1 amino terminus, arguing that the
      HCF-1 beta-propeller domain must target other cellular transcription factors in
      order to contribute to G(1) progression.
FAU - Mahajan, S S
AU  - Mahajan SS
AD  - Department of Microbiology, Kaplan Comprehensive Cancer Center, New York
      University School of Medicine, New York, New York 10016, USA.
FAU - Wilson, A C
AU  - Wilson AC
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Mol Cell Biol
JT  - Molecular and cellular biology
JID - 8109087
RN  - 0 (CREB3 protein, human)
RN  - 0 (Cyclic AMP Response Element-Binding Protein)
RN  - 0 (HCFC1 protein, human)
RN  - 0 (Herpes Simplex Virus Protein Vmw65)
RN  - 0 (Host Cell Factor C1)
RN  - 0 (Proteins)
RN  - 0 (Recombinant Proteins)
RN  - 0 (Transcription Factors)
SB  - IM
MH  - Amino Acid Sequence
MH  - Amino Acid Substitution
MH  - Cell Cycle/*physiology
MH  - Cell Division
MH  - Cell Line
MH  - Cyclic AMP Response Element-Binding Protein
MH  - G1 Phase
MH  - Herpes Simplex Virus Protein Vmw65/*metabolism
MH  - Host Cell Factor C1
MH  - Humans
MH  - Leucine Zippers
MH  - Molecular Sequence Data
MH  - Mutagenesis, Site-Directed
MH  - Protein Structure, Secondary
MH  - Proteins/chemistry/*genetics/*metabolism
MH  - Recombinant Proteins/chemistry/metabolism
MH  - Repetitive Sequences, Amino Acid
MH  - Sequence Alignment
MH  - Sequence Homology, Amino Acid
MH  - Simplexvirus/*genetics/metabolism
MH  - Transcription Factors/*metabolism
MH  - Transfection
PMC - PMC85209
EDAT- 2000/01/11 09:00
MHDA- 2000/02/19 09:00
CRDT- 2000/01/11 09:00
PHST- 2000/01/11 09:00 [pubmed]
PHST- 2000/02/19 09:00 [medline]
PHST- 2000/01/11 09:00 [entrez]
AID - 10.1128/MCB.20.3.919-928.2000 [doi]
PST - ppublish
SO  - Mol Cell Biol. 2000 Feb;20(3):919-28. doi: 10.1128/MCB.20.3.919-928.2000.