PMID- 10627548
OWN - NLM
STAT- MEDLINE
DCOM- 20000207
LR  - 20190508
IS  - 0022-538X (Print)
IS  - 0022-538X (Linking)
VI  - 74
IP  - 3
DP  - 2000 Feb
TI  - Productive measles virus brain infection and apoptosis in CD46 transgenic mice.
PG  - 1373-82
AB  - Measles virus (MV) infection causes acute childhood disease, associated in
      certain cases with infection of the central nervous system (CNS) and development 
      of neurological disease. To develop a murine model of MV-induced pathology, we
      generated several lines of transgenic mice ubiquitously expressing as the MV
      receptor a human CD46 molecule with either a Cyt1 or Cyt2 cytoplasmic tail. All
      transgenic lines expressed CD46 protein in the brain. Newborn transgenic mice, in
      contrast to nontransgenic controls, were highly sensitive to intracerebral
      infection by the MV Edmonston strain. Signs of clinical illness (lack of
      mobility, tremors, and weight loss) appeared within 5 to 7 days after infection, 
      followed by seizures, paralysis, and death of the infected animals. Virus
      replication was detected in neurons from infected mice, and virus was
      reproducibly isolated from transgenic brain tissue. MV-induced apoptosis observed
      in different brain regions preceded the death of infected animals. Similar
      results were obtained with mice expressing either a Cyt1 or Cyt2 cytoplasmic
      tail, demonstrating the ability of different isoforms of CD46 to function as MV
      receptors in vivo. In addition, maternally transferred immunity delayed death of 
      offspring given a lethal dose of MV. These results document a novel CD46
      transgenic murine model where MV neuronal infection is associated with the
      production of infectious virus, similarly to progressive infectious measles
      encephalitis seen in immunocompromised patients, and provide a new means to study
      pathogenesis of MV infection in the CNS.
FAU - Evlashev, A
AU  - Evlashev A
AD  - INSERM U503, Immunobiologie Fondamentale et Clinique, ENS de Lyon, Lyon, France.
FAU - Moyse, E
AU  - Moyse E
FAU - Valentin, H
AU  - Valentin H
FAU - Azocar, O
AU  - Azocar O
FAU - Trescol-Biemont, M C
AU  - Trescol-Biemont MC
FAU - Marie, J C
AU  - Marie JC
FAU - Rabourdin-Combe, C
AU  - Rabourdin-Combe C
FAU - Horvat, B
AU  - Horvat B
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Virol
JT  - Journal of virology
JID - 0113724
RN  - 0 (Antigens, CD)
RN  - 0 (CD46 protein, human)
RN  - 0 (Mcp protein, mouse)
RN  - 0 (Membrane Cofactor Protein)
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (Receptors, Virus)
SB  - IM
MH  - Animals
MH  - Antigens, CD/genetics/metabolism
MH  - Apoptosis
MH  - Brain/metabolism/*pathology/virology
MH  - Disease Models, Animal
MH  - Encephalitis, Viral/*pathology/virology
MH  - Female
MH  - Humans
MH  - Immunity, Maternally-Acquired
MH  - Measles/immunology/*pathology/virology
MH  - Measles virus/isolation & purification/*physiology
MH  - Membrane Cofactor Protein
MH  - Membrane Glycoproteins/genetics/metabolism
MH  - Mice
MH  - Mice, Transgenic
MH  - Pregnancy
MH  - Receptors, Virus/genetics/metabolism
MH  - Transgenes
MH  - Virus Replication
PMC - PMC111472
EDAT- 2000/01/11 00:00
MHDA- 2000/01/11 00:01
CRDT- 2000/01/11 00:00
PHST- 2000/01/11 00:00 [pubmed]
PHST- 2000/01/11 00:01 [medline]
PHST- 2000/01/11 00:00 [entrez]
AID - 10.1128/jvi.74.3.1373-1382.2000 [doi]
PST - ppublish
SO  - J Virol. 2000 Feb;74(3):1373-82. doi: 10.1128/jvi.74.3.1373-1382.2000.