PMID- 10627540
OWN - NLM
STAT- MEDLINE
DCOM- 20000207
LR  - 20190508
IS  - 0022-538X (Print)
IS  - 0022-538X (Linking)
VI  - 74
IP  - 3
DP  - 2000 Feb
TI  - Avian endogenous retrovirus EAV-HP shares regions of identity with avian leukosis
      virus subgroup J and the avian retrotransposon ART-CH.
PG  - 1296-306
AB  - The existence of novel endogenous retrovirus elements in the chicken genome,
      designated EAV-HP, with close sequence identity to the env gene of avian leukosis
      virus (ALV) subgroup J has been reported (L. M. Smith, A. A. Toye, K. Howes, N.
      Bumstead, L. N. Payne, and K. Venugopal, J. Gen. Virol. 80:261-268, 1999). To
      resolve the genome structure of these retroviral elements, we have determined the
      complete sequence of two proviral clones of EAV-HP from a line N chicken genomic 
      DNA yeast artificial chromosome library and from a meat-type chicken line 21
      lambda library. The EAV-HP sequences from the two lines were 98% identical and
      had a typical provirus structure. The two EAV-HP clones showed identical large
      deletions spanning part of the gag, the entire pol, and part of the env genes.
      The env region of the EAV-HP clones was 97% identical to the env sequence of
      HPRS-103, the prototype subgroup J ALV. The 5' region of EAV-HP comprising the R 
      and U5 regions of the long terminal repeat (LTR), the untranslated leader, and
      the 5' end of the putative gag region were 97% identical to the avian
      retrotransposon sequence, ART-CH. The remaining gag sequence shared less than 60%
      identity with other ALV sequences. The U3 region of the LTR was distinct from
      those of other retroviruses but contained some of the conserved motifs required
      for functioning as a promoter. To examine the ability of this endogenous
      retroviral LTR to function as a transcriptional promoter, the EAV-HP and HPRS-103
      LTR U3 regions were compared in a luciferase reporter gene assay. The low
      luciferase activity detected with the EAV-HP LTR U3 constructs, at levels close
      to those observed for a control vector lacking the promoter or enhancer elements,
      suggested that these elements function as a weak promoter, possibly accounting
      for their low expression levels in chicken embryos.
FAU - Sacco, M A
AU  - Sacco MA
AD  - Institute for Animal Health, Compton, Newbury, Berkshire RG20 7NN, United
      Kingdom.
FAU - Flannery, D M
AU  - Flannery DM
FAU - Howes, K
AU  - Howes K
FAU - Venugopal, K
AU  - Venugopal K
LA  - eng
SI  - GENBANK/AJ238120
SI  - GENBANK/AJ238121
SI  - GENBANK/AJ238122
SI  - GENBANK/AJ238123
SI  - GENBANK/AJ238124
SI  - GENBANK/AJ238125
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Virol
JT  - Journal of virology
JID - 0113724
RN  - 0 (5' Untranslated Regions)
RN  - 0 (Retroelements)
RN  - 0 (Retroviridae Proteins)
SB  - IM
MH  - 5' Untranslated Regions/genetics
MH  - Amino Acid Motifs
MH  - Amino Acid Sequence
MH  - Animals
MH  - Avian Leukosis Virus/classification/*genetics
MH  - Base Sequence
MH  - Chick Embryo
MH  - Endogenous Retroviruses/*genetics
MH  - Gene Deletion
MH  - Genes, env/genetics
MH  - Genes, gag/genetics
MH  - Genes, pol/genetics
MH  - Molecular Sequence Data
MH  - Promoter Regions, Genetic
MH  - Proviruses/*genetics
MH  - Retroelements/*genetics
MH  - Retroviridae Proteins/chemistry/genetics
MH  - Sequence Alignment
MH  - Terminal Repeat Sequences/genetics
MH  - Transcription, Genetic
PMC - PMC111464
EDAT- 2000/01/11 00:00
MHDA- 2000/01/11 00:01
CRDT- 2000/01/11 00:00
PHST- 2000/01/11 00:00 [pubmed]
PHST- 2000/01/11 00:01 [medline]
PHST- 2000/01/11 00:00 [entrez]
AID - 10.1128/jvi.74.3.1296-1306.2000 [doi]
PST - ppublish
SO  - J Virol. 2000 Feb;74(3):1296-306. doi: 10.1128/jvi.74.3.1296-1306.2000.