PMID- 10625651
OWN - NLM
STAT- MEDLINE
DCOM- 20000218
LR  - 20210209
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 275
IP  - 2
DP  - 2000 Jan 14
TI  - Muscle develops a specific form of small heat shock protein complex composed of
      MKBP/HSPB2 and HSPB3 during myogenic differentiation.
PG  - 1095-104
AB  - Previously, we identified a new mammalian sHSP, MKBP, as a myotonic dystrophy
      protein kinase-binding protein, and suggested its important role in muscle
      maintenance (Suzuki, A., Sugiyama, Y., Hayashi, Y., Nyu-i, N., Yoshida, M.,
      Nonaka, I., Ishiura, S., Arahata, K., and Ohno, S. (1998) J. Cell Biol. 140,
      1113-1124). In this paper, we develop the former work by performing extensive
      characterization of five of the six sHSPs so far identified, that is, HSP27,
      alphaB-crystallin, p20, MKBP/HSPB2, and HSPB3, omitting lens-specific
      alphaA-crystallin. Tissue distribution analysis revealed that although each sHSP 
      shows differential constitutive expression in restricted tissues, tissues that
      express all five sHSPs are only muscle-related tissues. Especially, the
      expressions of HSPB3, identified for the first time as a 17-kDa protein in this
      paper, and MKBP/HSPB2 are distinctly specific to muscles. Moreover, these sHSPs
      form an oligomeric complex with an apparent molecular mass of 150 kDa that is
      completely independent of the oligomers formed by HSP27, alphaB-crystallin, and
      p20. The expressions of MKBP/HSPB2 and HSPB3 are induced during muscle
      differentiation under the control of MyoD, suggesting that the sHSP oligomer
      comprising MKBP/HSPB2 and HSPB3 represents an additional system closely related
      to muscle function. The functional divergence among sHSPs in different oligomers 
      is also demonstrated in several ways: 1) an interaction with myotonic dystrophy
      protein kinase, which has been suggested to be important for the maintenance of
      myofibril integrity, was observed only for MKBP/HSPB2; 2) a myotube-specific
      association with actin bundles was observed for HSP27 and alphaB-crystallin, but 
      not for MKBP/HSPB2; and 3) sHSPs whose mRNAs are induced by heat shock are
      alphaB-crystallin and HSP27. Taken together, the results suggest that muscle
      cells develop two kinds of stress response systems composed of diverged sHSP
      members, and that these systems work independently in muscle maintenance and
      differentiation.
FAU - Sugiyama, Y
AU  - Sugiyama Y
AD  - Department of Molecular Biology, Yokohama City University School of Medicine,
      Kanazawa-ku, Yokohama 236-0004, Japan.
FAU - Suzuki, A
AU  - Suzuki A
FAU - Kishikawa, M
AU  - Kishikawa M
FAU - Akutsu, R
AU  - Akutsu R
FAU - Hirose, T
AU  - Hirose T
FAU - Waye, M M
AU  - Waye MM
FAU - Tsui, S K
AU  - Tsui SK
FAU - Yoshida, S
AU  - Yoshida S
FAU - Ohno, S
AU  - Ohno S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (Carrier Proteins)
RN  - 0 (Crystallins)
RN  - 0 (HSP27 Heat-Shock Proteins)
RN  - 0 (HSPB2 protein, human)
RN  - 0 (HSPB3 protein, human)
RN  - 0 (Heat-Shock Proteins)
RN  - 0 (Hspb2 protein, mouse)
RN  - 0 (Hspb2 protein, rat)
RN  - 0 (RNA, Messenger)
RN  - 0 (Recombinant Proteins)
SB  - IM
MH  - Adult
MH  - Animals
MH  - Carrier Proteins/*genetics/*metabolism
MH  - Cell Differentiation
MH  - Cell Line
MH  - Crystallins/genetics
MH  - Female
MH  - Gene Expression Regulation
MH  - Gene Library
MH  - HSP27 Heat-Shock Proteins
MH  - Heat-Shock Proteins/*genetics/*metabolism
MH  - Humans
MH  - Male
MH  - Mammals
MH  - Mice
MH  - Muscle, Skeletal/cytology/*metabolism
MH  - Organ Specificity
MH  - RNA, Messenger/genetics
MH  - Rats
MH  - Recombinant Proteins/metabolism
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Sequence Alignment
MH  - Sequence Homology, Amino Acid
MH  - Transcription, Genetic
MH  - Transfection
EDAT- 2000/01/08 09:00
MHDA- 2000/02/26 09:00
CRDT- 2000/01/08 09:00
PHST- 2000/01/08 09:00 [pubmed]
PHST- 2000/02/26 09:00 [medline]
PHST- 2000/01/08 09:00 [entrez]
AID - 10.1074/jbc.275.2.1095 [doi]
AID - S0021-9258(18)31223-7 [pii]
PST - ppublish
SO  - J Biol Chem. 2000 Jan 14;275(2):1095-104. doi: 10.1074/jbc.275.2.1095.